The LPS is in an aqueous solution containing 0.5% triethylamine and pH adjusted to 6.5 using hydrochloric acid.
L5543
Lipopolysaccharides from Escherichia coli O26:B6
Ready Made solution, 1 mg/mL, 0.2 μm filtered
Synonym(s):
LPS
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|
About This Item
Pricing and availability is not currently available.
biological source
Escherichia coli (O26:B6)
Quality Segment
sterility
0.2 μm filtered
form
aqueous solution
concentration
1 mg/mL
color
colorless to faint yellow
solubility
water: soluble
shipped in
wet ice
storage temp.
2-8°C
General description
This product is a 0.2 um filtered aqueous solution containing 1 mg/mL of lipopolysaccharides (LPS) from E. coli serotype O26:B6. This LPS serotype has been used in studies of septic shock.
Application
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Biochem/physiol Actions
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Preparation Note
The LPS used to prepare the solution have been phenol-extracted and chromatographically purified by gel filtration. Lipopolysaccharides prepared by phenol extraction contain up to 60% RNA and less than 1% protein. Subsequent purification by gel filtration chromatography removes much of protein present in the phenol-extracted LPS, but leaves a product that still contains 10-20% nucleic acids. Further purification using ion exchange chromatography yields an LPS product which contains <1% protein and <1% RNA.
Other Notes
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
1 of 1
This Item | |||
|---|---|---|---|
| biological source Escherichia coli (O26:B6) | biological source Escherichia coli (O127:B8) | biological source Escherichia coli (O26:B6) | biological source Escherichia coli (O26:B6) |
| solubility water: soluble | solubility water: soluble | solubility water: soluble | solubility water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form aqueous solution | form buffered aqueous solution | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| color colorless to faint yellow | color colorless to faint yellow | color white to faint yellow | color white to faint yellow |
Still not finding the right product?
Explore all of our products under Lipopolysaccharides from Escherichia coli O26:B6
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
What buffer is L5543, LPS in ?
1 answer-
Helpful?
-