The UniProt accession for this product is P46406, and the expression system used is Glyceraldehyde-3-phosphate dehydrogenase.
G5262
GAPDH
standard for protein electrophoresis
Synonym(s):
Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle, D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-609-4
MDL number:
EC Number:
Pricing and availability is not currently available.
Quality Segment
grade
Molecular Biology
form
powder
mol wt
~36 kDa
packaging
vial of 5 mg
technique(s)
electrophoresis: suitable
storage temp.
2-8°C
General description
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway. GAPDH has also been found to function in additional cellular process, such as transcription, apoptosis, oxidative stress and ER to Golgi transport.
Application
GAPDH protein is suitable for use as a molecular weight marker and protein standard for molecular biology applications, including western blotting and mass spectometry.
Biochem/physiol Actions
Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway.
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This Item | |||
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| description standard for protein electrophoresis | description recombinant, expressed in Escherichia coli | description ~1 mg/mL, affinity isolated antibody, buffered aqueous solution | description from chicken, purified by affinity chromatography |
| grade Molecular Biology | grade - | grade - | grade - |
| technique(s) electrophoresis: suitable | technique(s) - | technique(s) immunoprecipitation (IP): 5-10 μg using mouse NIH3T3 cell lysates, indirect immunofluorescence: 5-10 μg/mL using rat NRK cells, western blot: 0.1-0.2 μg/mL using whole extract of human HeLa cells | technique(s) western blot: suitable |
| form powder | form - | form buffered aqueous solution | form - |
| mol wt ~36 kDa | mol wt - | mol wt antigen ~36 kDa | mol wt - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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What is the uniprot accession and expression system used (if any)? Thanks.
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