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Sigma-Aldrich

Adenosine 5′-triphosphate (ATP) assay mix

lyophilized powder

Synonym(s):

ATP assay kit, ATP detection kit

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160414
NACRES:
NA.84

form

lyophilized powder

Quality Level

usage

1 vial sufficient for ≥50 assays

technique(s)

PCR: suitable

storage temp.

−20°C

General description

The Adenosine 5’-triphosphate (ATP) Bioluminescent Assay Kit may be employed for the quantitative bioluminescent determination of adenosine 5’-triphosphate (ATP) in samples containing 2x10–12 to 8x10–5 moles/liter. ATP is consumed and light is emitted when luciferase catalyzes the oxidation of D-luciferin.

Application

Adenosine 5′-triphosphate (ATP) assay mix has also been used to measure the level of cellular ATP in ATP viability assay.
The ATP assay kit has been used to measure the level of cellular ATP present in isolated mitochondrial fractions from L. donovani promastigotes.

Components

Contains luciferase, luciferin, MgSO4, EDTA, DTT, BSA, and Tricine buffer.

Reconstitution

Reconstitute with 5 ml of sterile water.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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