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Fluorescein 5(6)-isothiocyanate

Name: Fluorescein 5(6)-isothiocyanate
Brand: SIGMA

≥90% purity (HPLC), powder

Synonym(s):

FITC, Fluorescein isothiocyanate

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About This Item

Empirical Formula (Hill Notation):

C₂₁H₁₁NO₅S

CAS Number:

27072-45-3

Molecular Weight:

389.38

NACRES:

NA.47

PubChem Substance ID:

24894855

UNSPSC Code:

12171500

EC Number:

248-207-7

MDL number:

MFCD00005063

Beilstein/REAXYS Number:

1407295

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Properties

Product Name

Fluorescein 5(6)-isothiocyanate, ≥90% (HPLC)

Quality Segment

200

assay

≥90% (HPLC)

form

powder

technique(s)

titration: suitable

color

yellow to orange

solubility

acetone: 1 mg/mL, clear, light yellow to yellow

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1

InChI

1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H

InChI key

MHMNJMPURVTYEJ-UHFFFAOYSA-N

Description

General description

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.

Application

For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography

Fluorescein 5(6)-isothiocyanate has been used:

for fluorescence labeling of bacteria
as a standard for calibration of fluorometer
for the labeling of exosomes

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This Item	F4274	46950	F7250
Sigma-Aldrich F3651 Fluorescein 5(6)-isothiocyanate	Sigma-Aldrich F4274 Fluorescein isothiocyanate isomer I	Sigma-Aldrich 46950 Fluorescein 5(6)-isothiocyanate	Sigma-Aldrich F7250 Fluorescein isothiocyanate isomer I
description ≥90% (HPLC)	description ≥97.5% (HPLC)	description BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)	description suitable for protein labeling, ≥90% (HPLC), powder
technique(s) titration: suitable	technique(s) titration: suitable	technique(s) -	technique(s) titration: suitable
color yellow to orange	color yellow to very dark yellow	color yellow to dark yellow, orange to dark orange	color orange to dark orange
application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) -	application(s) diagnostic assay manufacturing hematology histology
solubility acetone: 1 mg/mL, clear, light yellow to yellow	solubility acetone: soluble 10 mg/mL	solubility acetone: 0.1 g/10 mL, clear to very slightly hazy	solubility acetone: 1 mg/mL
form powder	form powder	form powder	form powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C

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Storage Class

11 - Combustible Solids

Pictograms

GHS07

Signal Word

Warning

Hazard Codes

H302,H315,H317

Precautionary Statements

P261 - P264 - P272 - P280 - P301 + P312 - P302 + P352

Hazard Classifications

Acute Tox. 4 Oral - Skin Irrit. 2 - Skin Sens. 1B

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