DFF100

DEAE–Sepharose^™

Name: DEAE–Sepharose<SUP>™</SUP>
Brand: SIGMA

Fast Flow

Synonym(s):

Diethylaminoethyl–Sepharose^™

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About This Item

CAS Number:

57407-08-6

UNSPSC Code:

47101511

NACRES:

NA.56

MDL number:

MFCD00146630

Key Documents

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Product Name

DEAE–Sepharose^™, Fast Flow

form

suspension

technique(s)

affinity chromatography: suitable

matrix

6% cross-linked agarose

bead size

45-165 μm (wet)

pore size

~4,000,000 Da exclusion limit

pH

2—12

capacity

110-160 μeq/mL binding capacity(gel volume)

Quality Level

200

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Show Differences

1 of 4

This Item	A50120	DCL6B100	CCF100
Sigma-Aldrich DFF100 DEAE–Sepharose^™	Sigma-Aldrich A50120 DEAE–Sephadex^®	Sigma-Aldrich DCL6B100 DEAE–Sepharose^™	Sigma-Aldrich CCF100 CM Sepharose^™
pore size ~4,000,000 Da exclusion limit	pore size ~200,000 Da exclusion limit	pore size ~4,000,000 Da exclusion limit	pore size 4,000,000 exclusion limit (av. mol. wt.)
matrix 6% cross-linked agarose	matrix cross-linked dextran	matrix 6% cross-linked agarose	matrix 6% cross-linked agarose
technique(s) affinity chromatography: suitable	technique(s) protein purification: suitable	technique(s) affinity chromatography: suitable	technique(s) affinity chromatography: suitable
capacity 110-160 μeq/mL binding capacity(gel volume)	capacity 3-4 meq/g binding capacity	capacity 130-170 μeq/mL binding capacity (gel volume)(gel volume)	capacity 90-130 μeq/mL(gel basis)
form suspension	form powder	form suspension	form suspension
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

Application

DEAE-Sepharose has been used in:

anion exchange chromatography
to screen polyisoprenyl phosphate phosphatase activity[1]
to purify isoinhibitors[2]
for the purification of human immunodeficiency virus (HIV)
glycoprotein envelope (gp140 env)[3]

DEAE-Sepharose^™ is used in affinity chromatography, protein chromatography and ion exchange chromatography. DEAE-Sepharose^™ has been used to study pathogenesis of human disease and to develop a new assay for detecting the toxins of pathogenic strains of Clostridium difficile.

Biochem/physiol Actions

Diethylaminoethyl-sepharose (DEAE-sepharose) is a strong anion exchange column, where DEAE covalently binds to sepharose.[4]

General description

DFF100-500Ml′s update product number is GE17-0709-01

Legal Information

Sepharose is a trademark of Cytiva

comparable product

Product No.

Description

Pricing

GE17-0709-01

DEAE Sepharose^™ Fast Flow, Cytiva 17-0709-01, pack of 500 mL

pictograms

GHS02

signalword

Warning

hcodes

H226

pcodes

P210 - P233 - P240 - P241 - P242 - P243

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk

WGK 1

flash_point_f

100.4 - 109.4 °F

flash_point_c

38 - 43 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Role of Two Metacaspases in Development and Pathogenicity of the Rice Blast Fungus Magnaporthe oryzae.

Jessie Fernandez et al.

mBio, 12(1) (2021-02-11)

Rice blast disease caused by Magnaporthe oryzae is a devastating disease of cultivated rice worldwide. Infections by this fungus lead to a significant reduction in rice yields and threats to food security. To gain better insight into growth and cell

Metals and activity of bovine heart cytochrome c oxidase are independent of polypeptide subunits III, VII, a, and b.

G L Yewey et al.

Biochemical and biophysical research communications, 148(3), 1520-1526 (1987-11-13)

Bovine heart cytochrome c oxidase, depleted of polypeptide subunits by alkaline detergent treatment, was characterized with respect to metal content, optical spectral properties, and oxidase activity. Treatment with 1.0% Triton X-100 at pH 9.5 followed by anion-exchange chromatography caused removal

Advances in Potato Chemistry and Technology (2016)

Bioluminescence of the insect pathogen Xenorhabdus luminescens.

T M Schmidt et al.

Applied and environmental microbiology, 55(10), 2607-2612 (1989-10-01)

Luminescence of batch cultures of Xenorhabdus luminescens was maximal when cultures approached stationary phase; the onset of in vivo luminescence coincided with a burst of synthesis of bacterial luciferase, the enzyme responsible for luminescence. Expression of luciferase was aldehyde limited

Rapid high-level production of functional HIV broadly neutralizing monoclonal antibodies in transient plant expression systems

Rosenberg Y, et al.

Testing, 8(3), e58724-e58724 (2013)

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DEAE–Sepharose™