biological source
Rat x Rat Hybridoma
ImmunoDon
Rat DA spleen
Myeloma
Y3
Reactivity
Recombinant CD147 domain-0
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Description
The hybridoma cell line OX-115 recognises an alternatively spliced domain of mouse CD147.
Immunogen
Recombinant CD147
Application
The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)
Subculture Routine
Seed at 5 x 104 cells/ml (5 x 105 cells/ml at resuscitation). Subculture when cells reach 5 x 105 - 1 x 106 cells/ml (usually after 3-4 days), 5% CO2, 37°C. 20% FBS can be used at resuscitation.
Other Notes
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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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