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C5914

Cell Dissociation Solution Non-enzymatic 1x

Prepared in phosphate buffered saline without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

Dissociation Solution

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.75
MDL number:
MFCD00282844
Concentration:
1 ×
Pricing and availability is not currently available.
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Quality Segment

200

sterility

sterile-filtered

product line

BioReagent

form

liquid

concentration

1 ×

technique(s)

cell culture | mammalian: suitable

pH

6.9-7.1

shipped in

wet ice

storage temp.

2-8°C

General description

Cell dissociation by non-enzymatic methods in human mesenchymal stromal cells aid in their detachment and preserves their functionality especially the expression of cell surface chemokine receptor.

Application

Special non-enzymatic formulation for gently dislodging adherent cell types from plastic or glass surfaces.
Cell Dissociation Solution Non-enzymatic 1x has been used for the dissociation of retinal astrocytes culture, U87 glioblastoma tumor cells and cardiac stem cells (CSCs).

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flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

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Questions

1–6 of 6 Questions  

  1. How does the product C5914 dissociate the cells from culture dishes etc?

    1 answer

    1. The formulation includes EDTA and sodium citrate, which chelate or sequester divalent cations, thereby disrupting the calcium (Ca²⁺) and magnesium (Mg²⁺) bridges responsible for cell–cell adhesion.

      Helpful?

  2. Can one use Product C5914, Cell Dissociation Solution, with tissue?

    1 answer

    1. We have only used with monolayer cell culture, and therefore we have no information on its usage with tissue.

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  3. Does Product C5914, Cell Dissociation Solution, work with epithelial cells?

    1 answer

    1. It will work with most cell types.

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  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. Does Product C5914, Cell Dissociation Solution, contain EDTA?

    1 answer

    1. Yes, it does contain EDTA; the concentration is proprietary.

      Helpful?

  6. When trypsin is used to detach cells, one usually adds fetal bovine serum (FBS) to the media. Should FBS be used with Product C5914, Cell Dissociation Solution?

    1 answer

    1. No. FBS is used to inactivate trypsin. This product is not enzymatic, so there is no need to use FBS. The cells can be washed with phosphate buffered saline (PBS). When the cells are to be cultured, then one would need to add FBS.

      Helpful?

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