C4859

Cycloheximide solution

Ready-Made Solution, microbial, 100 mg/mL in DMSO, Suitable for cell culture

• Synonym(s): CHX
• Empirical Formula (Hill Notation): C₁₅H₂₃NO₄
• CAS Number: 66-81-9
• Molecular Weight: 281.35
• NACRES: NA.77
• PubChem Substance ID: 24892732
• UNSPSC Code: 12352111
• MDL number: MFCD00082346
• Form: ready-to-use solution

Properties

• biological source: microbial
• Quality Segment: 300
• description: suitable for testing specifications (CHO C. growth inhibitor)
• form: ready-to-use solution
• concentration: 100 mg/mL in DMSO
• solubility: DMSO: soluble
• antibiotic activity spectrum: fungi, yeast
• mode of action: protein synthesis | interferes
• storage temp.: 2-8°C
• SMILES string: O=C1[C@@]([C@H](O)CC2CC(NC(C2)=O)=O)([H])C[C@@H](C)C[C@@H]1C
• InChI: 1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1
• InChI key: YPHMISFOHDHNIV-FSZOTQKASA-N
• Gene Information: human ... FKBP1A(2280), PIN1(5300)

Description

Application

Cycloheximide solution has been used:

• to study its role in modulating cell death induced by tumor necrosis factor α (TNFα) in combination with the second mitochondria-derived activator of caspases (SMAC) mimetic birinapant or interferon-gamma (IFNγ), specifically under conditions that inhibit caspase-8 activity[1]
• in the study to inhibit protein translation during the CHX-chase assays[2]
• to treat antisense oligonucleotide (AON) transfected patient-derived fibroblasts and inhibit the nonsense-mediated decay (NMD) pathway to analyze the therapeutic potential AON and visualize the transcript containing the pseudoexon (PE)[3]

Biochem/physiol Actions

Cycloheximide (CHX) is an antibiotic produced by S. griseus. Its main biological activity is translation inhibition in eukaryotes resulting in cell growth arrest and cell death. CHX is widely used for selection of CHX-resistant strains of yeast and fungi, controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression, and apoptosis induction or facilitation of apoptosis induction by death receptors.

Tested for cell growth arrest, selection of cycloheximide resistant yeast, apoptosis induction, and facilitation of apoptosis induction by FasL.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.Hygroscopic. Storage class (TRGS 510): Non-combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials.

Safety Information

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H301,H315,H319,H341,H360D,H412
• pcodes: P202 - P264 - P273 - P301 + P310 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Muta. 2 - Repr. 1B - Skin Irrit. 2
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• flash_point_f: 188.6 °F - closed cup
• flash_point_c: 87 °C - closed cup