The molecular weight for this product has not been determined in-house. Other sources have reported the molecular weight to be 484.7 g/mol.
C0543
Mouse Collagen Type IV
from Engelbreth-Holm-Swarm murine sarcoma basement membrane, lyophilized powder, suitable for cell culture, used for 3D hydrogel formation
Synonym(s):
Type IV collagen, EHS-collagen
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About This Item
Biological source:
mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)
Form:
lyophilized powder
Technique(s):
cell culture | mammalian: suitable
Pricing and availability is not currently available.
Product Name
Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane, Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture
biological source
mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)
Quality Segment
type
Type IV (Miller)
product line
BioReagent
form
lyophilized powder
packaging
glass bottle of 1 vial
technique(s)
cell culture | mammalian: suitable
surface coverage
6‑10 μg/cm2
solubility
0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)
shipped in
dry ice
storage temp.
−20°C
Gene Information
mouse ... Col4a1(12826), Col4a2(12827), Col4a3(12828), Col4a5(12830), Col4a6(94216)
General description
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.
Collagen is classified into several structurally and genetically distinct types. Type IV collagen is a predominant structural protein of the basement membrane. It is notably characteristic of the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma. The target cells for attachment include: epithelial cells, endothelial cells, muscle cells, and nerve cells.
Application
Recommended for use as a cell culture substratum at 6-10 μg/cm2.
Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used:
- in adhesion assays.
- in hydrogel fabrication.
- to coat cell culture plates for cell differentiation.
- to treat round plastic coverslips for scanning electron microscopy (SEM) analysis in an interaction study between A. culbertsoni trophozoites and Schwann cells.
Biochem/physiol Actions
Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules. It is also used in 2D coatings.
Preparation Note
0.75 mg/vial
Chloroform treated.
Pepsin is not used in the preparation.
Pepsin is not used in the preparation.
Other Notes
Native collagen
We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma-type designations with recognized collagen classification types.
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This Item | |||
|---|---|---|---|
| biological source mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane) | biological source human cell culture | biological source rat tail | biological source rat tail |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form solution | form liquid | form powder |
| solubility 0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.) | solubility - | solubility soluble, clear to hazy, colorless (solubilized in 0.02M acetic acid) | solubility soluble |
| Gene Information mouse ... Col4a1(12826), Col4a2(12827), Col4a3(12828), Col4a5(12830), Col4a6(94216) | Gene Information human ... COL4A1(1282) | Gene Information rat ... Col1a1(29393) | Gene Information rat ... Col1a1(29393) |
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Could you provide me with the observed molecular weight of the Rat Collagen Type IV product from the Engelbreth-Holm-Swarm murine sarcoma basement membrane? CAS Number:9007-34-5
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