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5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

Name: 5-Bromo-4-chloro-3-indolyl &#946;-<SC>D</SC>-galactopyranoside
Brand: SIGMA

≥98%, powder, suitable for identification of lac+ bacterial colonies

Synonym(s):

5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal

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About This Item

Empirical Formula (Hill Notation):

C₁₄H₁₅BrClNO₆

CAS Number:

7240-90-6

Molecular Weight:

408.63

NACRES:

NA.83

PubChem Substance ID:

57653968

UNSPSC Code:

12352204

EC Number:

230-640-8

MDL number:

MFCD00005666

Beilstein/REAXYS Number:

1402009

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Properties

Product Name

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, ≥98%

Quality Segment

200

assay

≥98%

form

powder

solubility

DMF: methanol (1:1): soluble 50 mg/mL

suitability

suitable for identification of lac⁺ bacterial colonies

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI key

OPIFSICVWOWJMJ-AEOCFKNESA-N

Description

Application

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac⁺ genotype.

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside has been used to confirm transduction of Caki-2/LacZ clones. It has also been used in blue-white selection of transformants.

Preparation Note

A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20 °C and protected from light. Sterilization is not required.

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This Item	B4252	B6024	203782
Sigma-Aldrich B9146 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside	Sigma-Aldrich B4252 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside	Sigma-Aldrich B6024 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside	Sigma-Aldrich 203782 5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside
assay ≥98%	assay ≥98%	assay -	assay ≥99% (HPLC)
solubility DMF: methanol (1:1): soluble 50 mg/mL	solubility DMF: methanol (1:1): 50 mg/mL	solubility -	solubility DMSO: 2% (20mg/mL)
suitability suitable for identification of lac⁺ bacterial colonies	suitability -	suitability suitable for identification of lac⁺ bacterial colonies	suitability -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100
form powder	form powder	form tablet	form solid
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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