B7151
Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat
affinity isolated antibody, buffered aqueous solution
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About This Item
Conjugate:
biotin conjugate
Clone:
polyclonal
Application:
ELISA (d), IHC (p), WB
Citations:
48
biological source
goat
Quality Level
conjugate
biotin conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:200,000-1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot: 1:400,000-1:800,000 using total cell extract of HeLa cells
storage temp.
−20°C
target post-translational modification
unmodified
General description
IgG (immunoglobulin G) antibody subtype is a predominant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. IgG is found in four subclasses IgG1, IgG2, IgG3 and IgG4. IgG has a monomeric structure.
The product binds to all mouse Igs
Application
Anti-Mouse IgG (Fab specific)-Biotin antibody produced in goat was used in immunohistochemistry of lateral and medial menisci of rabbits and human adipose-derived stem cells.
Biochem/physiol Actions
IgG (immunoglobulin G) antibody participates in complement fixation and opsonization.
IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to Anti-Mouse IgG (Fab specific) antibody allows for the binding of various labels such as avidin or streptavidin.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Adsorbed to reduce background staining with bovine, horse or human samples.
Other Notes
Antibody adsorbed with bovine, equine and human serum proteins.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Brian O Diekman et al.
Journal of biomedical materials research. Part A, 93(3), 994-1003 (2009-09-02)
Adipose-derived stem cells (ASCs) are multipotent progenitors that can be chondrogenically induced by growth factors such as bone morphogenetic protein 6 (BMP-6). We hypothesized that nonviral transfection of a BMP-6 construct (pcDNA3-BMP6) would induce chondrogenic differentiation of ASCs encapsulated in
Franklin T Moutos et al.
Tissue engineering. Part A, 16(4), 1291-1301 (2009-11-12)
Articular cartilage possesses complex mechanical properties that provide healthy joints the ability to bear repeated loads and maintain smooth articulating surfaces over an entire lifetime. In this study, we utilized a fiber-reinforced composite scaffold designed to mimic the anisotropic, nonlinear
G M Cunniffe et al.
European cells & materials, 33, 130-142 (2017-02-16)
Limitations associated with demineralised bone matrix and other grafting materials have motivated the development of alternative strategies to enhance the repair of large bone defects. The growth plate (GP) of developing limbs contain a plethora of growth factors and matrix
Global Trade Item Number
| SKU | GTIN |
|---|---|
| B7151-1ML | 04061837514913 |
| B7151-.5ML | 04061837514906 |