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B4186

Butyrylcholinesterase human

vial of ≥4 units

Synonym(s):

Acylcholine acyl-hydrolase, Choline esterase, butyryl, Pseudocholinesterase

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About This Item

CAS Number:
9001-08-5
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-579-2
MDL number:
MFCD00081455
EC Number:
3.1.1.8 (BRENDA, IUBMB)
Specific activity:
≥50 units/mg protein
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form

powder

Quality Level

200

specific activity

≥50 units/mg protein

packaging

vial of ≥4 units

UniProt accession no.

P06276

storage temp.

2-8°C

Gene Information

human ... BCHE(590)

Application

BchE, from Sigma, has been used to determine butyrylcholinesterase inhibition by various test compounds [1].
Butyrylcholinesterase human has been used to study its role as a modulator of pathological features of Alzheimer′s disease (AD) in vivo.

Biochem/physiol Actions

Butyrylcholinesterase (BChE) is a serine hydrolase that shares substantial structural similarities with acetylcholinesterase (AchE) but has different substrate and inhibitor specificities. BChE is found in the serum, hemopoietic cells, liver, lung, heart and the central nervous system of vertebrates.
Butyrylcholinesterase (BChE) is a serine hydrolase that shares substantial structural similarities with acetylcholinesterase (AchE) but has different substrate and inhibitor specificities[2]. It is found in the serum, hemopoietic cells, liver, lung, heart and the central nervous system of vertebrates. BChE hydrolyzes large esters of choline such as butyrylcholine and benzoylcholine. Selective inhibition of BChE activity can be used in the detection of organophosphates. BchE may be used to treat organophosphate toxicity. It is a critical target for Alzheimer′s disease (AD) therapy.

Analysis Note

Starting material individually donor tested and found negative for HIV I & II antibodies, Hepatitis B surfaceantigen, and Hepatitis C antibodies.

Other Notes

One unit will hydrolyze 1.0 μmole of butyrylcholine to choline and butyrate per min at pH 8.0 at 37 °C. The activity obtained using butyrylcholine as substrate is ~2.5 times that obtained using acetylcholine.

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This Item
C1057C7512C1682
Butyrylcholinesterase human vial of ≥4 units

Sigma-Aldrich

B4186

Butyrylcholinesterase human

Butyrylcholinesterase from equine serum lyophilized powder, ≥900 units/mg protein

Sigma-Aldrich

C1057

Butyrylcholinesterase from equine serum

Butyrylcholinesterase from equine serum lyophilized powder, ≥10 units/mg protein

Sigma-Aldrich

C7512

Butyrylcholinesterase from equine serum

Acetylcholinesterase human recombinant, expressed in HEK 293 cells, lyophilized powder, ≥1,000 units/mg protein (BCA)

Sigma-Aldrich

C1682

Acetylcholinesterase human

Gene Information

human ... BCHE(590)

Gene Information

-

Gene Information

-

Gene Information

human ... ACHE(43)

specific activity

≥50 units/mg protein

specific activity

≥900 units/mg protein

specific activity

≥10 units/mg protein

specific activity

≥1,000 units/mg protein (BCA)

form

powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

UniProt accession no.

P06276

UniProt accession no.

-

UniProt accession no.

-

UniProt accession no.

P22303

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000534063
0000513947
0000513947
0000534063
0000501787

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Butyrylcholinesterase: an important new target in Alzheimer's disease therapy
Greig NH, et al.
International Psychogeriatrics / IPA, 14(S1), 77-91 (2002)
Cholinesterases and engineered mutants for the detection of organophosphorus pesticide residues
Xu YL, et al.
Sensors, 18(12), 4281-4281 (2018)
The apolipoprotein E varepsilon4 allele plays pathological roles in AD through high protein expression and interaction with butyrylcholinesterase
Darreh-Shori T, et al.
Neurobiology of Aging, 32(7), 1236-1248 (2011)
View All Related Papers

Global Trade Item Number

SKUGTIN
B4186-1VL04061833429372

Questions

1–5 of 5 Questions  

  1. What is the stability of Butyrylcholinesterase human vial of ≥4 units when stored at recommended temperature of 2-8C?

    1 answer

    1. This product is not assigned an expiration or retest date. Products that are robust in nature and extremely stable, in the original unopened container, are not assigned an expiration date or retest schedule. These products will have no dating reported on either the label or the Certificate of Analysis.

      Products that are not assigned an expiration or retest date are covered by a warranty period of 1 year from the date of product shipment.

      Full Product Dating Information may be reviewed at the link below: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/356/113/product-dating-information-06-25-ms.pdf

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. What amount of enzyme is present in 1 vial of Product B4186, Butyrylcholinesterase from human serum?

    1 answer

    1. Each vial will contain at least 5 units. The vial content is given on the certificate of analysis of each lot. The vial may be substantially overpacked.

      Helpful?

  4. How should Product B4186, Butyrylcholinesterase from human serum, be reconstituted?

    1 answer

    1. The enzyme can be reconstituted in 20 mM sodium phosphate buffer, pH 7.0, with 0.15 M sodium chloride.

      Helpful?

  5. Are any buffer salts present in Product B4186, Butyrylcholinesterase from human serum?

    1 answer

    1. This product was lyophilized from 20 mM ammonium bicarbonate, which is a volatile buffer. So it would be expected to contain only traces of buffer salts.

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