AB0300

Alkaline Phosphatase Blue Membrane Substrate Solution

alkaline phosphatase substrate, solution

• UNSPSC Code: 12352204
• NACRES: NA.83
• eCl@ss: 42010105

Properties

• Product Name: Alkaline Phosphatase Blue Membrane Substrate Solution, sufficient for 400 mL working substrate
• form: solution
• Quality Segment: 200
• usage: sufficient for 400 mL working substrate
• storage temp.: 2-8°C

Description

General description

Alkaline phosphatase, a metalloenzyme is a member of ectonucleotidases family. It is present in bile ducts, bone, intestine, placenta and tumors.

Application

Prior to reaction with alkaline phosphatase, the BCIP^® reagent is colorless to faint blue and the Nitro BT reagent is yellow. The two-component mixture develops a bluish-purple product with alkaline phosphatase in membrane-type assays. This substrate is not recommended for ELISA assays.

Alkaline Phosphatase Blue Membrane Substrate Solution has been used:

• to analyse direct alkaline phosphatase (AP) activity
• to perform alkaline phosphatase assays with colorimetric system
• to analyse direct alkaline phosphatase (AP) activity of the induced pluripotent stem cells (iPSC) line LND554SV.3

Physical form

A 2 component kit sufficient for preparing 400 mL of working substrate.

Supplied as a two-component buffered alkaline phosphatase substrate containing bromochloroindolyl phosphate (BCIP^®) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable