Alkaline Phosphatase Blue Microwell Substrate

Name: Alkaline Phosphatase Blue Microwell Substrate
Brand: SIGMA

alkaline phosphatase substrate, solution

Synonym(s):

5-bromo-4-chloro-3-indolyl phosphate analog / NBT substrate for microwell assays

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.83

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Properties

Product Name

Alkaline Phosphatase Blue Microwell Substrate, sufficient for 400 mL working substrate

form

solution

Quality Segment

200

usage

sufficient for 400 mL working substrate

storage temp.

2-8°C

Description

General description

Alkaline phosphatase blue microwell substrate, a two-component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require an insoluble reaction product.

Application

Prior to reaction with alkaline phosphatase, the BCIP^® reagent is colorless to faint blue solution, and the Nitro BT reagent is a yellow solution. The two component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require a precipitation reaction product.

Physical form

A 2 component kit sufficient for preparing 400 mL of working substrate.

Supplied as a 2 component buffered alkaline phosphatase substrate containing Bromo-Chloro-Indolylphosphate (BCIP) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)