This product can be reconstituted in ammonium hydroxide, pH >9, to a minimum of 1 mg/mL, or in DMSO at 0.4–4 mM. Stock solutions can then be serially diluted into aqueous buffers, such as PBS or saline, to the desired working concentrations. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdf
A9810
Amyloid β Protein Fragment 1-42
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About This Item
Empirical Formula (Hill Notation):
C203H311N55O60S
CAS Number:
Molecular Weight:
4514.04
UNSPSC Code:
12352202
NACRES:
NA.32
MDL number:
Pricing and availability is not currently available.
assay
≥95% (HPLC)
Quality Segment
form
powder
UniProt accession no.
storage temp.
−20°C
SMILES string
S(CC[C@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(
InChI
1S/C203H311N55O60S/c1-28-106(20)164(195(310)220-91-149(267)228-130(71-98(4)5)181(296)238-129(66-70-319-27)179(294)251-158(100(8)9)193(308)218-87-146(264)215-88-151(269)250-160(102(12)13)198(313)255-163(105(18)19)199(314)258-165(107(21)29-2)200(315)227-112
InChI key
DZHSAHHDTRWUTF-SIQRNXPUSA-N
Gene Information
human ... APP(351)
General description
Amyloid β Protein is produced from amyloid-β precursor protein (APP). It consists of two C terminal variants, such as a long tailed Aβ 1-42 and a short tailed Aβ 1-40. APP is located on human chromosome 21q21.3.
Application
Amyloid β Protein Fragment 1-42 has been used:
- for the preparation of Aβ 1-42 oligomer[1]
- for western blot analysis
- for the interference test of immunomagnetic reduction (IMR) plasma Aβ42 assay
- to study the effects of resveratrol on Aβ 1-42-induced impairment of spatial learning, memory and synaptic plasticity
- to investigate the effect of Aβ in epithelial cell culture
Biochem/physiol Actions
The predominant fragment of amyloid β-protein found in the brains of patients with Alzheimer′s disease and Down′s syndrome.
Amyloid β Protein Fragment 1-42 (Aβ 1-42) has antioxidant and neuroprotective properties. Accumulation of amyloid β Protein is associated with Alzheimer′s disease (AD) and Down Syndrome. Aβ 1-42 regulates cholesterol transport and may function as a transcription factor. It may possess anti-inflammatory and antimicrobial properties.
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This Item | |||
|---|---|---|---|
| assay ≥95% (HPLC) | assay ≥95% (HPLC) | assay ≥90% (HPLC) | assay >97.0% |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| form powder | form powder | form powder | form (Clear dry film) |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| Gene Information human ... APP(351) | Gene Information human ... APP(351) | Gene Information human ... APP(351) | Gene Information human ... APP(351) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A9810-.1MG | 04061835553754 |
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What would be the best method to reconstitute this amyloid β protein fragment 1-42 into the concentrations starting at 0.1nM and working my way up to 5, 10 and 100nM?
1 answer-
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After dissolving in DMSO, I want to dilute it with saline (finally 1ml/1mg) and use it for i.c.v. injection in mice. How many µl of 0.4-4 mM DMSO per 0.1 mg should I dissolve in?
1 answer-
This product is soluble in DMSO at 0.4 - 4 mM, as stated in the product datasheet. This equates to 1.8 - 18 mg/mL in DMSO. This stock solution may be diluted with aqueous buffers, such as PBS or saline, to the desired working concentration. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdfHelpful?
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What is the difference between the AB peptide (1-42)(PP69) and AB protein fragment (1-42)(A9810)?
1 answer-
These products are functionally the same, but are different brands. The PP69 is a Calbiochem product, while the A9810 is from Sigma-Aldrich. The testing specifications are slightly different, however both offer a minimum purity of 95%. The A9810 is lyophilized from a solution of water and TFA. The PP69 is lyophilized from a solution of 50 mM Tris-HCl. Please see the links below to review the product datasheet for each:
PP69 Calbiochem:
https://www.emdmillipore.com/product/-Amyloid-Peptide-1-42-Human,EMD_BIO-PP69#anchor_PDS
A9810 Sigma-Aldrich:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdfHelpful?
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It is instructed to dissolve the product in Ammonium Hydroxide solution, but what should be the molarity / Concentration of the ammonium hydroxide solution
1 answer-
This product can be solubilized in ammonium hydroxide at a pH greater than 9. The concentration is not reported. It is recommended to prepare at a minimum 1 mg/mL concentration.
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How much ammonium hydroxide (pH >9) should be added to the 0,1 mg vial ? Is it possible to vortex the vial ?
1 answer-
It is recommended to prepare a solution at a minimum of 1 mg/mL of ammonium hydroxide. Vertexing the vial is not necessary.
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in which solvent it should be dissolved ?
1 answer-
As per the product information sheet, this product is soluble in ammonium hydroxide, pH >9, or in DMSO at 0.4–4 mM. Solutions can then be diluted into aqueous buffers such as10 mM sodium phosphate for use. Other reported solvents include hexafluoroisopropanol, acetonitrile, and acetic acid.
See the link below to review the product information sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/301/154/a9810pis.pdfHelpful?
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What is the pI of Product A9810, Amyloid β Protein Fragment 1-42?
1 answer-
The pI as calculated from the amino acid sequence for Product A9810, Amyloid β Protein Fragment 1-42, is 5.31.
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What is the source of Product A9810, Amyloid β Protein Fragment 1-42?
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This protein fragment is a synthetic organic. The gene for this peptide is located on human chromosome 21.
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Is the mouse protein fragment the same as the human protein fragment in Product A9810, Amyloid β Protein Fragment 1-42?
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The homology is 93% by identity, 95% positives... no gaps
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What is the secondary structure of Product A9810, Amyloid β Protein Fragment 1-42?
1 answer-
The secondary structure of this fragment will exist as a mixture of B-sheet, a-helix, and random coil structures dependent upon the solution conditions as described in J. Mol. Biol., 225, 1075-1083, 1992
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