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A2220

ANTI-FLAG® M2 Affinity Gel

purified immunoglobulin, buffered aqueous glycerol solution

Synonym(s):

Monoclonal ANTI-FLAG® M2, ANTI-FLAG® M2 Affinity Agarose Gel, Anti-ddddk, Anti-dykddddk

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352203
Conjugate:
agarose conjugate
Clone:
M2, monoclonal
Application:
IP, affinity chromatography
Citations:
2532
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conjugate

agarose conjugate

Quality Segment

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous glycerol solution

analyte chemical class(es)

proteins

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

matrix

(4% agarose bead; 45-165μm bead size)

isotype

IgG1

capacity

>0.6 mg/mL, resin binding capacity (FLAG-BAP)

shipped in

wet ice

storage temp.

−20°C

General description

Anti-FLAG M2 Affinity gel is a mouse monoclonal antibody that is covalently attached to agarose. The antibody binds FLAG at the N-terminal, Met-N-terminal, C-terminal and internal locations of fusion proteins. Binding is calcium-independent.

Elution - FLAG® peptide, Glycine, pH 3.5, 3x FLAG® peptide
FLAG® peptide, Glycine, pH3.5, 3x FLAG® peptide

Immunogen

DYKDDDDK

Application

Anti-FLAG® M2 affinity gel has been used for western blotting, immunoprecipitation and for the purification of FLAG fusion proteins.

Learn more product details in our FLAG® application portal.

Physical form

Suspension in buffered saline containing azide as preservative and 50% glycerol

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

FLAG® tag, 3x FLAG®, DYKDDDDK tag

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F2426A9469F3165
description

purified immunoglobulin, buffered aqueous glycerol solution

description

clone M2

description

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

description

clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

conjugate

agarose conjugate

conjugate

-

conjugate

alkaline phosphatase conjugate

conjugate

unconjugated

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

antibody form

purified immunoglobulin

antibody form

-

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin (Purified IgG1 subclass)

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): suitable, affinity chromatography: suitable

technique(s)

indirect ELISA: 1:20,000

technique(s)

western blot: 10 μg/mL (Protein A)

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C


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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable



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