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91228

Sigma-Aldrich

Trichloroacetic acid

BioUltra, ≥99.5% (T)

Synonym(s):

TCA

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About This Item

Linear Formula:
Cl3CCOOH
CAS Number:
Molecular Weight:
163.39
Beilstein:
970119
EC Number:
MDL number:
UNSPSC Code:
12352101
PubChem Substance ID:
NACRES:
NA.25

vapor density

<1 (vs air)

Quality Level

vapor pressure

1 mmHg ( 51 °C)

product line

BioUltra

Assay

≥99.5% (T)

form

crystals
flakes

impurities

insoluble matter, passes filter test

ign. residue

≤0.05%

refractive index

n20/D 1.62 (lit.)

pH

<1.0 (25 °C, 0.5 M in H2O)

bp

196 °C (lit.)

mp

54-58 °C (lit.)

solubility

H2O: 0.5 M at 20 °C, clear, colorless

density

1.62 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤30 mg/kg
nitrate (NO3-): ≤20 mg/kg
phosphate (PO43-): ≤10 mg/kg
sulfate (SO42-): ≤100 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

SMILES string

OC(=O)C(Cl)(Cl)Cl

λ

0.5 M in H2O

UV absorption

λ: 260 nm Amax: 1.60
λ: 280 nm Amax: 0.15

InChI

1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)

InChI key

YNJBWRMUSHSURL-UHFFFAOYSA-N

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General description

Trichloroacetic acid (TCA) is derived from Trichloroethylene (TCE) metabolism. It is used as an acid decalcifying agent. TCA is used as a fixative for nuclear staining and protein precipitation.

Application

Trichloroacetic acid (TCA) has been used:
  • to determine its effect on avian embryos during an exposure window encompassing cardiac specification
  • as a cell fixative
  • to terminate the O-acetylserine (thiol)lyase (OASTL) enzymatic assay
  • for the precipitation of acidic ribosomal proteins (RPs)

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.

Other Notes

Concentration of protein by quantitative precipitation; Cytochemical demonstration of DNA with a thiazine dye

Pictograms

CorrosionEnvironment

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

>235.4 °F - closed cup

Flash Point(C)

> 113 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Extraction and Activity of O-acetylserine (thiol) lyase (OASTL) from Microalga Chlorella sorokiniana
Salbitani G and Carfagna S
Bio-protocol (2016)
M.K. Dutt
Science and Culture, 51, 196-196 (1985)
Histophatologic Techniques (2006)
Cardiogenic effects of trichloroethylene and trichloroacetic acid following exposure during heart specification of avian development
Drake V, et al.
Toxicological Sciences, 94(1), 153-162 (2006)
Efficacy of 5-FU or oxaliplatin monotherapy over combination therapy in colorectal cancer
Toloudi M, et al.
Journal of Cancer Therapy, 6(04), 345-345 (2015)

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