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Tetramethylrhodamine isothiocyanate mixed isomers

Name: Tetramethylrhodamine isothiocyanate mixed isomers
Brand: SIGMA

suitable for fluorescence, mixture of isomers

Synonym(s):

MRITC, TRITC, Tetramethylrhodamine B isothiocyanate

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About This Item

Empirical Formula (Hill Notation):

C₂₅H₂₁N₃O₃S

CAS Number:

95197-95-8

Molecular Weight:

443.52

MDL number:

MFCD00002867

NACRES:

NA.32

UNSPSC Code:

12352116

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Properties

Quality Segment

200

form

powder

solubility

DMSO: soluble, methanol: soluble

fluorescence

λ_ex 529 nm; λ_em 596 nm in DMSO

suitability

suitable for fluorescence

storage temp.

2-8°C

Description

General description

Tetramethylrhodamine isothiocyanate, also known as TRITC is a fluorescent antibody labelling agent. It is a red-fluorescing marker and can be tracked through fluorescence microscopy.[1]

TRITC dissolves completely in DMSO at a rapid rate. And gamma globulin is unaffected to up to 30% DMSO dilution in carbonate buffer. Tetramethylrhodamine isothiocyanate doesn’t dissolve in other denaturing agents completely.[2]

Application

Tetramethylrhodamine isothiocyanate or TRITC is used for tracking the pathway of calcium phosphate/DNA nanoparticles during cell transfection. [1]It is an extremely intensive fluorochrome used for labeling antibodies.

Other Notes

An extremely intensive fluorochrome for labeling antibodies[3]

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This Item	T3163	52194	42874
Sigma-Aldrich 87918 Tetramethylrhodamine isothiocyanate mixed isomers	Sigma-Aldrich T3163 Tetramethylrhodamine isothiocyanate Isomer R	Sigma-Aldrich 52194 Tetramethylrhodamine isothiocyanate–Dextran	Sigma-Aldrich 42874 Tetramethylrhodamine isothiocyanate–Dextran
suitability suitable for fluorescence	suitability -	suitability -	suitability -
fluorescence λ_ex 529 nm; λ_em 596 nm in DMSO	fluorescence -	fluorescence λ_ex 550 nm; λ_em 575 nm±5 nm in 0.1 M Tris pH 8.0	fluorescence λ_ex 550 nm; λ_em 577 nm±5 nm in 0.1 M Tris pH 8.0
solubility DMSO: soluble, methanol: soluble	solubility 1 M NH4OH: 10 mg/mL (Faint Red to Very Dark Red to Very Dark Purple solution)	solubility -	solubility -
form powder	form powder	form powder or crystals	form powder
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 100
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. -	storage temp. -

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H317,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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