56822

Immersion oil

suitable for microscopy

• Synonym(s): oil immersion medium for light microscopy
• UNSPSC Code: 12171500
• NACRES: NA.47
• MDL number: MFCD00131369

Properties

• biological source: synthetic
• Quality Segment: 100
• form: liquid
• refractive index: n20/D 1.516
• viscosity: 100-120 mPa.s(20 °C)
• density: 1.025 g/mL at 20 °C
• suitability: suitable for microscopy
• application(s): hematology; histology
• storage temp.: room temp

Description

General description

Immersion oil is a clear, viscous liquid with optimized refractive properties, specifically modified to closely approximate the refractive index (RI) of glass (ne = 1.5). It is used in conjunction with an objective lens to enhance the resolving power.

Application

Immersion oil is used for high-resolution (1000X) light microscopy work in conjunction with an oil immersion objective lens to optimize microscopic examinations of histological, cytological, hematological, and bacterial specimen material after it has been fixed, embedded, stained, or counterstained, and mounted.

Biochem/physiol Actions

Immersion oil is applied dropwise to stained and mounted or non-mounted specimen material to form a clear film between the specimen and the microscope lens to eliminate the deflection of incident light and thus substantially enhance the optical efficiency of the lens.

Features and Benefits

• Immersion oil for general use in light microscopy.
• Excellent, defined refractive index eliminates refractive distortion.
• Engineered for optimal viscosity and homogeneity.
• Good UV transmission and low residual fluorescence.

Safety Information

• pictograms: GHS09
• signalword: Warning
• hcodes: H410
• pcodes: P273 - P391 - P501
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 2
• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type ABEK (EN14387) respirator filter