M1428

MIO Medium

NutriSelect^® Plus, suitable for microbiology

• Synonym(s): Motility Indole Ornithine Medium
• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• product line: BioChemika
• form: powder
• shelf life: limited shelf life, expiry date on the label
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbe id | growth & motility: suitable; microbe id | specific enzyme detection: suitable; microbiological culture: suitable
• final pH: 6.5±0.2 (25 °C)
• application(s): clinical testing; environmental; food and beverages; veterinary; microbiology
• suitability: Escherichia coli; nonselective and differential for Enterobacter spp.; nonselective and differential for Klebsiella spp.; nonselective and differential for Proteus spp.; nonselective and differential for enterobacteriaceae

Description

Application

Used for the identification of enterobacteriaceae on the basis of motility, indole production and ornithine decarboxylase activity.

Components

Ingredients (g/L)
Casein enzymatic hydrolysate, 10.00
Peptic digest of animal tissue, 10.00
Yeast extract, 3.00
L-Ornithine hydrochloride, 5.00
Dextrose, 1.00
Bromocresol purple, 0.02
Agar, 2.00

Preparation Note

Suspend 31 g of MIO Medium in 1000 ml of distilled water. Boil to dissolve the medium completely. Dispense 5 ml into each test tube. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool the tubes in an upright position.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves