E7889

Ethylenediaminetetraacetic acid disodium salt solution

Molecular Biology, 0.5 M in H₂O, DNase, RNase, NICKase and protease, none detected

• Synonym(s): (Ethylenedinitrilo)tetraacetic acid disodium salt, EDTA disodium salt, EDTA-Na₂
• Linear Formula: [-CH₂N(CH₂CO₂Na)CH₂CO₂H]₂
• CAS Number: 139-33-3
• Molecular Weight: 336.21
• UNSPSC Code: 12352107
• NACRES: NA.31
• PubChem Substance ID: 24894660
• MDL number: MFCD00070672
• Beilstein/REAXYS Number: 3822669

Properties

• grade: Molecular Biology
• Quality Segment: 300
• sterility: 0.2 μm filtered
• form: liquid
• reaction suitability: reagent type: chelator
• concentration: 0.5 M in H₂O
• pH: 7.5-8.5
• suitability: suitable for molecular biology
• application(s): microbiology
• foreign activity: DNase, RNase, NICKase and protease, none detected
• SMILES string: [Na+].[Na+].OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
• InChI: 1S/C10H16N2O8.2Na/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;/q;2*+1/p-2
• InChI key: ZGTMUACCHSMWAC-UHFFFAOYSA-L

Description

General description

Ethylenediaminetetraacetic acid (EDTA) is a polyprotic acid. The four carboxylic acid and two amine groups with lone-pair electrons of EDTA can chelate calcium and several other metal ions.

Ethylenediaminetetraacetic acid disodium salt solution is a chelating agent suitable for molecular biology applications.

Application

Ethylenediaminetetraacetic acid disodium salt solution has been used:

• in Tris hydrochloric acid (HCl) for digesting proteinase-K to determine the amount of DNA in the corneal sample
• with papain to digest corneal samples for the quantification of glycosaminoglycan (GAG) content
• in pretreatment for detaching epithelial cells grown to 80% confluency
• to prepare stock solutions for lysis buffer
• in the preparation of urea lysis buffer

Features and Benefits

molecular biology grade

Preparation Note

Prepared in 18 megohm water with Molecular Biology Reagent EDTA (E 5134)

Other Notes

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Safety Information

• pictograms: GHS08
• signalword: Warning
• hcodes: H373
• pcodes: P260 - P314 - P501
• Hazard Classifications: STOT RE 2 Inhalation
• target_organs: Respiratory Tract
• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable