Yes, DMSO is a suitable solvent for this material. Stock solutions are typically prepared at 10 mM, however concentrations of up to 20 mg/mL have been achieved.
Other solvents include Dimethylformamide, Chloroform, Acetonitrile, and Methanol. Stock solutions may be store in aliquots at -20 °C. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/401/542/d7008pis.pdf
D7008
Dihydroethidium
≥95% purity, powder
Synonym(s):
2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine, Hydroethidine
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About This Item
Empirical Formula (Hill Notation):
C21H21N3
CAS Number:
Molecular Weight:
315.41
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
Beilstein/REAXYS Number:
5107482
Pricing and availability is not currently available.
Product Name
Dihydroethidium, ≥95%
Quality Segment
assay
≥95%
form
powder
technique(s)
titration: suitable
color
pink to very dark red
solubility
chloroform: 20 mg/mL
fluorescence
λex 370 nm; λem 420 nm (for cytoplasm of living cells), λex 535 nm; λem 610 nm (for chromatin of living cells)
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
−20°C
SMILES string
CCN1C(c2ccccc2)c3cc(N)ccc3-c4ccc(N)cc14
InChI
1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3
InChI key
XYJODUBPWNZLML-UHFFFAOYSA-N
Application
Redox indicator. Blue fluorescence until oxidized to ethidium.
Dihydroethidium has been used as a superoxide probe in bovine pulmonary artery endothelial cell (BPAEC). It has been used to check the in situ generation of ROS (reactive oxygen species).[1]
Packaging
Packaged under Argon.
Other Notes
B-ring reduced analog of ethidium
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This Item | |||
|---|---|---|---|
| technique(s) titration: suitable | technique(s) - | technique(s) titration: suitable | technique(s) - |
| color pink to very dark red | color - | color white to orange | color faint orange to very dark orange |
| fluorescence λex 370 nm; λem 420 nm (for cytoplasm of living cells), λex 535 nm; λem 610 nm (for chromatin of living cells) | fluorescence λex 358 nm; λem 461 nm, λex 392 nm; λem 410 nm in methanol | fluorescence - | fluorescence λex 428 nm; λem 540 nm in H2O |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| solubility chloroform: 20 mg/mL | solubility acetonitrile: soluble, methanol: soluble | solubility H2O: 50 mg/mL | solubility H2O: 1 mg/mL |
| form powder | form solid | form powder | form powder |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Could DMSO be used as an alternative solvent?
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