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Basic Fuchsin

Name: Basic Fuchsin
Brand: SIAL

certified by the Biological Stain Commission, powder

Synonym(s):

Basic Red 9, Fuchsin basic, Magenta O, Pararosaniline

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About This Item

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24891537

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Properties

Product Name

Basic Fuchsin, special for flagella, certified

agency

certified by the BSC

Quality Segment

200

type

special for flagella, certified

form

powder

composition

pararosaniline acetate, 3 parts , pararosaniline chloride, 1 part

technique(s)

microbe id | staining: suitable

color

green to very dark green

pH

5.0-6.0 (1 g/L)

mp

250 °C

solubility

H₂O: 1 mg/mL

density

1.220 g/cm³

λ_max

544 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Description

General description

Basic fuchsin, a triphenylmethane dye is commonly a mixture of pararosaniline (C.I. 42500), rosaniline (C.I. 42510), magenta II and new fuchsine (CI 42520). Basic fuchsin is widely used in tissue-specific staining of biological and coloring several materials including orlon, cotton, leather, paper etc. It is also used to stain collagen, muscle and mitochondria.

Application

Basic Fuchsin has been used in several biological tests including:

Acid-fast staining with carbol-fuchsine
Periodic Acid-Schiff method
Feulgen technique for DNA staining
ENDO culture medium for distinguishing between the bacteria Escherichia coli and Aerobacter aerogenes
Leifson′s method for bacterial flagella staining.

Certified for use in Leifson′s flagella stain.

Biochem/physiol Actions

Basic fuchsin possesses anesthetic, bactericidal (gram-positive), and fungicidal properties.

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This Item	857343	P3750	F8129
Sigma-Aldrich B0904 Basic Fuchsin	Sigma-Aldrich 857343 Basic Fuchsin	Sigma-Aldrich P3750 Pararosaniline hydrochloride	Sigma-Aldrich F8129 Acid Fuchsin
description special for flagella, certified	description certified by the BSC, Dye content ≥88 %	description >85.0% (HPLC)	description used in tissue staining
technique(s) microbe id \| staining: suitable	technique(s) microbe id \| staining: suitable	technique(s) -	technique(s) microbe id \| staining: suitable
color green to very dark green	color green to dark green	color green to very dark green	color blue-green
application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology
form powder	form powder	form powder or crystals	form powder
solubility H₂O: 1 mg/mL	solubility -	solubility ethanol: 1 mg/mL	solubility H₂O: 1 mg/mL
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

pictograms

GHS08

signalword

Danger

hcodes

H350

pcodes

P201 - P308 + P313

Hazard Classifications

Carc. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P2 (EN 143) respirator cartridges

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