None of the methylene blue products are considered API or suitable for use in manufacturing medical devices. They are offered for Research Use Only or for In Vitro Diagnostic Testing. Methylene blue is offered as methylene blue chloride and as a methylene blue zinc chloride double salt, each with slightly different formulas and molecular weights. Methylene blue chloride is the form of the dye used in most biological staining applications and medicinal uses. It is recommended to source methylene blue from approved pharmaceutical suppliers, especially considering the availability of methylene blue USP grade.
28514
Methylene Blue hydrate
≥95% (calc. to the dried substance)
Synonym(s):
Basic Blue 9 monohydrate ; Methylene Blue (hydrate)
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About This Item
Empirical Formula (Hill Notation):
C16H18ClN3S · xH2O
CAS Number:
Molecular Weight:
319.85 (anhydrous basis)
UNSPSC Code:
12171500
NACRES:
MA.02
PubChem Substance ID:
EC Number:
200-515-2
Beilstein/REAXYS Number:
3599847
MDL number:
Pricing and availability is not currently available.
Quality Segment
assay
≥95% (calc. to the dried substance)
form
powder
impurities
≤1% insoluble in ethanol
loss
≤22% loss on drying, 105 °C
color
very dark green
solubility
ethanol: insoluble ≤1%
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C
InChI
1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1
InChI key
WQVSELLRAGBDLX-UHFFFAOYSA-M
General description
Methylene blue is bis(dimethylamino)phenothiazine hydrochloride (tetramethylthionine),
C16H18N3SCl. It is a major basic histological dye in pharmacology and microbiology. This heterocyclic aromatic chemical appears as a dark green solid powder, changing to blue in an aqueous solution. Methylene blue is tested for histological and and bacteriological staining, and as a constituent of Wright’s blood stain.
C16H18N3SCl. It is a major basic histological dye in pharmacology and microbiology. This heterocyclic aromatic chemical appears as a dark green solid powder, changing to blue in an aqueous solution. Methylene blue is tested for histological and and bacteriological staining, and as a constituent of Wright’s blood stain.
Application
Methylene blue is widely used in several routine staining applications including Mallory’s phloxine-methylene blue on paraffin sections of tissue fixed in Zenker’s fluid for nuclear staining and bacterial staining in milk. It is also suitable for manufacturing the polychrome methylene blue used in the preparation of Romanowsky stains.Methylene Blue hydrate has also been used:
- in the preparation of methylene blue (MB) solution
- to stain zymograms -to label the injection site in the tissue slices
- to pre-treat mice via oral gavage to study its effects on noise-induced hearing loss
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This Item | |||
|---|---|---|---|
| description ≥95% (calc. to the dried substance) | description suitable for nucleic acid staining, BioReagent | description meets analytical specification of BP 73 | description purum p.a., suitable for microscopy, ≥97.0% (calc. based on dry substance, AT) |
| color very dark green | color - | color - | color green to dark green |
| form powder | form powder | form powder | form powder |
| solubility ethanol: insoluble ≤1% | solubility water: 1 mg/mL (dark blue) | solubility - | solubility ethanol: 0.01 g/10 mL, blue to very deep blue |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) hematology | application(s) - |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| assay ≥95% (calc. to the dried substance) | assay - | assay - | assay ≥97.0% (calc. based on dry substance, AT) |
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Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
dust mask type N95 (US), Eyeshields, Gloves
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Are any of the methylene blue products considered API or suitable for use in manufacturing medical devices? The products offered for sale are for Research Use Only or for In Vitro Diagnostic Testing? Additionally, what are the differences between methylene blue chloride and methylene blue zinc chloride double salt offered?
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