RSTREP-RO

Streptavidin, recombinant

liquid, ≥17 units/mg protein, pH range 5.0-9.0

• Synonym(s): streptavidin
• UNSPSC Code: 12352202

Properties

• biological source: Streptomyces sp. ( Streptomyces avidinii)
• Quality Level: 100
• recombinant: expressed in E. coli
• form: liquid
• specific activity: ≥17 units/mg protein
• mol wt: ~52 kDa
• packaging: pkg of 1 mg (11721666001); pkg of 5 mg (11721674001)
• manufacturer/tradename: Roche
• concentration: 70-90%
• technique(s): protein array: suitable; protein purification: suitable
• impurities: 6-7% water
• pH range: 5.0-9.0
• pI: 7.4-7.7 (two main bands)
• solubility: water: 10 mg/L (mA₄₀₅=100)
• suitability: suitable for immunological tests
• UniProt accession no.: P22629
• shipped in: cooler
• storage temp.: -10 to -25°C

Description

General description

Streptavidin, a highly versatile protein, is naturally produced by Streptomyces avidinii. It exhibits a strong affinity for biotin, forming a non-covalent yet robust binding interaction. This unique property makes streptavidin an ideal choice for various biological detection systems, including western blotting, enzyme-linked immunosorbent assays (ELISA), and immunohistochemistry.

Application

Use Streptavidin, recombinant for coating of solid phases (e.g., microplates, beads etc.) as well with biotin-labeled haptens and antibodies in universal immunological detection systems (streptavidin/biotin system).

Sequence

Streptavidin is a tetramer with a molecular weight of approximately 53 kD consisting of four subunits of approximately 13 kD, each containing a single biotin-binding site.
Each subunit has six tyrosine residues.The protein is free of carbohydrate.

Unit Definition

One unit binds 1 μg D-biotin at pH 8.9.

Physical form

White lyophilizate; lyophilized in a 20 mM potassium phosphate, pH 6.5 solution
Protein: 0.7 ± 0.1mg protein lyophilizate (A₂₈₂ = 3.1)
K_D: 10^-15M

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): does not flash
• Flash Point(C): does not flash