MABT166

Anti-Tom20/Tomm20 Antibody, clone 2F8.1

clone 2F8.1, from mouse

• Synonym(s): Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20, TOM20, TOMM20
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 2F8.1, monoclonal
• Application: ICC, WB
• Citations: 23

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2F8.1, monoclonal
• species reactivity: human, mouse
• technique(s): immunocytochemistry: suitable, western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_055580
• UniProt accession no.: Q15388
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TOMM20(9804)

Description

General description

Mitochondrial import receptor subunit TOM20 homolog (TOM20 or TOMM20), also known as mitochondrial 20 kDa outer membrane protein or outer mitochondrial membrane receptor Tom20, is a central component of the pre-protein translocase complex of the outer mitochondrial membrane (TOM complex) which is responsible for the recognition and translocation of cytosolically synthesized mitochondrial pre-proteins. The TOM complex consists of at least 7 different proteins (TOMM5, TOMM6, TOMM7, TOMM20, TOMM22, TOMM40 and TOMM70). TOM20 interacts with TOM22 and facilitates the movement of pre-proteins into the TOM40 translocation pore. Also interacts with APEX1.

~ 16 kDa observed

Immunogen

GST-tagged recombinant protein corresponding to human Tom20/Tomm20.

Application

Anti-Tom20/Tomm20, clone 2F8.1 detects levels of Tom20/Tomm20 proteins & has been published & validated for use in WB & ICC.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Tom20/Tomm20 in A431, NIH/3T3, and HeLa cells.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected Tom20/Tomm20 in 200 µg of HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable