MAB2160

Anti-Fragile X Mental Retardation Protein Antibody, clone 1C3

ascites fluid, clone 1C3, Chemicon^®

• Synonym(s): FMRP
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 1C3, monoclonal
• Application: ELISA, ICC, IHC (p), WB
• Citations: 73

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 1C3, monoclonal
• species reactivity: mouse, human, rat
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
• isotype: IgG1κ
• suitability: not suitable for flow cytometry, not suitable for immunoprecipitation
• NCBI accession no.: NM_002024.3
• UniProt accession no.: Q06787
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... FRAXA(2477), FRAXA(108684022)

Description

General description

Fragile X Mental Retardation Protein (FMRP) is a RNA-binding protein that is associated to polysomes and may be involved in the transport of mRNA from the nucleus to the cytoplasm. Defects in FMR1 are the cause of Fragile X syndrome, which is a common genetic disease characterized by moderate to severe mental retardation, macroorchidism, large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.

~71 kDa

Immunogen

Fusion protein with a full length FMRP (human).

Application

Anti-Fragile X Mental Retardation Protein Antibody, clone 1C3 detects level of Fragile X Mental Retardation Protein & has been published & validated for use in ELISA, IC, IH, IH(P) & WB.

Immunohistochemistry:
Frozen and paraffin sections. 1:500-1:5,000 dilution of a previous lot was used.

ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.

Detection of FMRP on Blood Smears:
A 1:500-1:5,000 dilution of a previous lot was used.

Immunocytochemistry:
A 1:500-1:5,000 dilution of a previous lot was used on transfected cells. Light fixation (2% PFA, permeabilize with 0.1% triton in block only)

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

RNA Binding Protein (RBP)

Biochem/physiol Actions

Human and mouse FMRP. The epitope is localized in the N-terminal half of FMRP. Cross reaction with FXR protein may be detected in cases of high expression of the latter proteins.

Physical form

Ascites mouse monoclonal IgG1k fluid containing no preservatives

Unpurified

Preparation Note

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
HeLa whole cell lysate, HeLa nuclear lysate, mouse E17 spinal cord lysate

Evaluated by Western Blot on Mouse E17 spinal cord lysates.

Western Blotting Analysis:
1:500 dilution of this antibody detected Fragile X Mental Retardation Protein on 10 μg of Mouse E17 spinal cord lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable