MAB1959Z
Anti-Integrin β1 Antibody, clone P5D2 (Azide Free)| MAB1959
clone P5D2, Chemicon®, from mouse
Synonym(s):
CD29, MAB1959
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
P5D2, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ITGB1(3688)
General description
Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.
Specificity
Reacts with Human Integrin Beta 1.
Immunogen
Human keratinocytes derived from skin.
Application
Immunoprecipitation: A representative lot of this antibody clone was used in immunoprecipitation.
Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry (acetone fixation, no paraffin embedding).
ELISA: A representative lot of this antibody was used in ELISA.
Immunocytochemistry: A representative lot of this antibody clone was used in immunocytochemistry (paraformaldehyde fixation at less than 4%).
Functional Activity Assay: A representative lot of this antibody clone was used in cell attachment assay of SV-HFO cells with a characteristic spread morphology. In the presence of function-blocking mAbs to β1 integrin (P5D2), the cells attached but no longer spread, and displayed a rounded morphology with many cytoplasmic projections (Iba, K. et al., 2000).
Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry (acetone fixation, no paraffin embedding).
ELISA: A representative lot of this antibody was used in ELISA.
Immunocytochemistry: A representative lot of this antibody clone was used in immunocytochemistry (paraformaldehyde fixation at less than 4%).
Functional Activity Assay: A representative lot of this antibody clone was used in cell attachment assay of SV-HFO cells with a characteristic spread morphology. In the presence of function-blocking mAbs to β1 integrin (P5D2), the cells attached but no longer spread, and displayed a rounded morphology with many cytoplasmic projections (Iba, K. et al., 2000).
Anti-Integrin β1 Antibody, clone P5D2 (Azide Free) detects level of Integrin β1 & has been published & validated for use in ELISA, FC, IC, IH, IP, FUNC.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
Quality
Flow Cytometry Analysis:
4 µg of the antibody was used to detect Integrin β1 in 1x10^6 A431 cells.
4 µg of the antibody was used to detect Integrin β1 in 1x10^6 HeLa cells.
4 µg of the antibody was used to detect Integrin β1 in 1x10^6 A431 cells.
4 µg of the antibody was used to detect Integrin β1 in 1x10^6 HeLa cells.
Physical form
Format: Purified
Protein A purified
Purified ascites in PBS without preservatives.
Storage and Stability
Store at -20°C in undiluted aliquots.
Avoid repeated freeze-thaw cycles.
Avoid repeated freeze-thaw cycles.
Analysis Note
Control
Human tonsil, human skin tissue
A431 & HeLa Cells
Human tonsil, human skin tissue
A431 & HeLa Cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
recommended
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Greeshma Thrivikraman et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(29) (2021-07-17)
Despite the ubiquitous importance of cell contact guidance, the signal-inducing contact guidance of mammalian cells in an aligned fibril network has defied elucidation. This is due to multiple interdependent signals that an aligned fibril network presents to cells, including, at
Lana Bruney et al.
Biological chemistry, 395(10), 1221-1231 (2014-09-11)
Mucin16 [MUC16/cancer antigen 125 (CA-125)], a high-molecular-weight glycoprotein expressed on the ovarian tumor cell surface, potentiates metastasis via selective binding to mesothelin on peritoneal mesothelial cells. Shed MUC16/CA-125 is detectable in sera from ovarian cancer patients. We investigated the potential
Human Enteric α-Defensin 5 Promotes Shigella Infection by Enhancing Bacterial Adhesion and Invasion.
Dan Xu et al.
Immunity, 48(6), 1233-1244 (2018-06-03)
Shigella is a Gram-negative bacterium that causes bacillary dysentery worldwide. It invades the intestinal epithelium to elicit intense inflammation and tissue damage, yet the underlying mechanisms of its host selectivity and low infectious inoculum remain perplexing. Here, we report that
Pablo Castro-Córdova et al.
Nature communications, 12(1), 1140-1140 (2021-02-20)
Clostridioides difficile spores produced during infection are important for the recurrence of the disease. Here, we show that C. difficile spores gain entry into the intestinal mucosa via pathways dependent on host fibronectin-α5β1 and vitronectin-αvβ1. The exosporium protein BclA3, on
Taru Muranen et al.
Nature communications, 8, 13989-13989 (2017-01-11)
Extracellular matrix adhesion is required for normal epithelial cell survival, nutrient uptake and metabolism. This requirement can be overcome by oncogene activation. Interestingly, inhibition of PI3K/mTOR leads to apoptosis of matrix-detached, but not matrix-attached cancer cells, suggesting that matrix-attached cells
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service