IF014

Interferon-β Protein, Recombinant human

Recombinant Human Interferon Beta 1a (Hu-IFNbeta 1a).

• Synonym(s): Fibroblast Interferon
• UNSPSC Code: 12352202
• NACRES: NA.77
• eCl@ss: 32160405
• Form: liquid
• Assay: ≥95% (one N-glycosylation site)
• Biological source: human
• Recombinant: expressed in CHO cells

Properties

• biological source: human
• Quality Segment: 100
• recombinant: expressed in CHO cells
• assay: ≥95% (one N-glycosylation site)
• form: liquid
• specific activity: 3.99 x 108 U/mg
• manufacturer/tradename: Chemicon^®
• concentration: 1 x 10⁶ units/mL
• technique(s): cell culture | mammalian: suitable
• NCBI accession no.: NM_002176.2
• UniProt accession no.: P01574
• shipped in: dry ice
• storage temp.: -65 to -96°C

Description

General description

Product Source: cDNA obtained from human fibroblast mRNA expressed in CHO cells.

Recombinant Human Interferon Beta 1a (Hu-IFNbeta 1a). Molecular weight is ~23 kDa.

Physical form

Frozen liquid in 50 mM NaOAc, pH 5.5, containing 0.1% BSA.

Preparation Note

After receipt, this product should be kept at -70°C or below for retention of full activity.
Thaw product vial by incubation cold tap water until just thawed. Thawed IFNβ should be
stored in working aliquots at -70°C for up to six months. Avoid multiple freeze/thaw cycles.
Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the
product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).

Analysis Note

Specific Activity: Determined by assaying inhibition of virus-induced cytopathic effect.In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%.The units are determined with respect to the international reference standard for human interferon beta (Hu-IFNbeta) provided by the National Institutes of Health.Unit of activity measured on VERO cells with vesicular stomatitis virus (VSV).

Other Notes

Replaces: IF008

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1