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CCYTOMAG-90K

Millipore

MILLIPLEX® Canine Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

Synonym(s):

canine interleukin immunoassay panel, luminex canine cytokine chemokine growth factor multiplex assay, luminex canine pro inflammatory anti inflammatory cytokine panel, millipore canine inflammation multiplex kit

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47
To order a Milliplex® kit, please search for your analyte of interest.
Design And Price Your Kit

Quality Level

200

species reactivity

canine

manufacturer/tradename

Milliplex®

assay range

accuracy: 91-109%
sensitivity: 13.6 pg/mL
(IFN-γ)
sensitivity: 21.0 pg/mL
(MCP-1)
sensitivity: 21.7 pg/mL
(IL-8)
sensitivity: 3.2 pg/mL
(IP-10)
sensitivity: 3.5 pg/mL
(IL-2)
sensitivity: 3.7 pg/mL
(IL-6)
sensitivity: 5.3 pg/mL
(KC-like)
sensitivity: 5.8 pg/mL
(IL-18)
sensitivity: 6.1 pg/mL
(TNFα)
sensitivity: 7.5 pg/mL
(IL-7)
sensitivity: 8.5 pg/mL
(IL-10)
sensitivity: 9.0 pg/mL
(IL-15)
sensitivity: 9.2 pg/mL
(GM-CSF)
standard curve range: 12.2-50,000 pg/mL
(All analytes)

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

In addition to studying inflammation for dog nutrition and disease research, canine animal models are important for human biomedical research and drug development. Cytokine and chemokines are molecular mediators of immune cell response in inflammation and immunity. Quantification of cytokines and chemokines is essential to understanding the immune system, as well as for researching disease states such as autoimmune, allergic reactions, sepsis, and cancer.

The MILLIPLEX® Canine Cytokine Panel is 13-plex kit to be used for the simultaneous quantification of any or all of the following analytes in canine serum, plasma, tissue/cell lysate and culture supernatant samples: GM-CSF, IFNγ, KC-like, IP-10, IL-2, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, MCP-1, and TNF-α. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Specificity

Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
There was no or negligible cross-reactivity among different analytes within the panel.

Application

  • Analytes: GM-CSF, IFNγ, IP-10, IL-2, IL-6, IL-7, IL-8, IL-10, IL-15, IL-18, KC-like, MCP-1, and TNF-α.
  • Recommended Sample Type: Canine serum, plasma, cell culture supernatant and tissue/cell lysates
  • Recommended Sample Dilution: 25 μL of a 1:3 dilution of plasma or serum, or 25 μL cell culture supernatant
  • Assay Run Time: Overnight at 4°C or 2 hours at room temperature (20-25° C)
  • Research Category: Inflammation & Immunology

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

Everything you need in a single kit.

Linkage

Replaces: CCYTO-90K

Storage and Stability

Recommended storage for kit components is 2 - 8°C

Other Notes

Please contact Technical Service for linearity of dilution.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chad B Maki et al.
Frontiers in veterinary science, 7, 570-570 (2020-10-29)
This study was conducted to investigate the therapeutic effect of allogeneic adipose-derived MSCs on dogs with hip osteoarthritis (OA). Twenty dogs with bilateral osteoarthritis of the coxofemoral (hip) joint, diagnosed by a veterinarian through physical examination and radiographs were randomly
J L Tuohy et al.
Journal of veterinary internal medicine, 30(4), 1167-1178 (2016-06-25)
Monocytes/macrophages are likely key cells in immune modulation in dogs with osteosarcoma (OSA). Increased peripheral monocyte counts are negatively correlated with shorter disease-free intervals in dogs with OSA. Understanding the monocyte/macrophage's modulatory role in dogs with OSA can direct further
Julien R S Dandrieux et al.
Veterinary medicine and science, 5(2), 199-205 (2019-01-22)
A pharmacodynamic assay has been previously developed to monitor ciclosporin treatment in dogs by assessing inhibition of cytokine transcription after whole blood stimulation with 12-myristate 13-1 acetate and ionomycin (PMA/I). In this study, whole blood stimulation with either PMA/I or
Bing Wang et al.
Acta biomaterialia, 116, 259-267 (2020-09-17)
In periodontal treatment, topical adjunctive therapy with antimicrobials or anti-inflammatory agents is frequently applied. However, currently available drug carrier biomaterials often exhibit poor perfusion into small crevices, such as the deep and irregular periodontal pockets, due to relatively high viscosity.
Gustavo Rodrigues Martins et al.
Mediators of inflammation, 2016, 9512743-9512743 (2016-03-19)
Inflammation results in the production of cytokines, such as interleukin- (IL-) 4 and IL-10 with immunosuppressive properties or IL-6 and TNF-α with procarcinogenic activity. Furthermore, NF-κB is the major link between inflammation and tumorigenesis. This study verified the interaction between
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Questions

  1. Are fecal samples compatible with Milliplex Immunoassays?

    1 answer

    1. Fecal contents are collected and weighed, then resuspended in PBS + 5% gelatin supplemented with a complete inhibitor cocktail. The samples are extracted with vortexing and prepared in a concentrated form to facilitate the detection of low levels of cytokines.

      The insoluble fraction is separated by centrifugation at greater than 10,000xg for 10 minutes at 4°C. The resulting supernatants can be assayed immediately or frozen at -80°C.

      It may be necessary to test an optimal dilution, using the extraction buffer as the assay matrix for standards and blanks.

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