In powder form, this product may be stored refrigerated at 2-8°C. Following reconstitution, aqueous solutions may be stored in aliquots at -20°C for extended periods. Historical data indicate solutions are stable at ambient or refrigerated temperatures for several weeks.
70663
Proteinase K, Lyophilized
Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA.
Synonym(s):
Proteinase K from Tritirachium album, Endopeptidase K
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About This Item
CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.85
EC Number:
MDL number:
eCl@ss:
32160410
Pricing and availability is not currently available.
Quality Segment
form
solid
mol wt
28.93 kDa
manufacturer/tradename
Novagen®
storage condition
OK to freeze
technique(s)
DNA extraction: suitable
shipped in
wet ice
storage temp.
2-8°C
General description
Proteinase K is a highly active 28,904-Daserine protease isolated from the fungusTritirachium album. The enzyme exhibitsbroad cleavage specificity on native anddenatured proteins and is widely used in thepurification of DNA and RNA. Its activityis increased in the presence of denaturantssuch as SDS (1%) and elevated temperature(50-60°C). The recommended workingconcentration is 50-100 µg/ml for proteinremoval and enzyme inactivation, and up to2 mg/ml for tissue treatment. The Proteinase K,Lyophilized powder can be prepared as a20 mg/ml stock solution in water and storedin aliquots at -20°C. The enzyme is alsoavailable as a ready-to-use concentrated stocksolution (600 mAU/ml) that is convenientfor routine use in most applications. 1 mgof Proteinase K is the equivalent of 30 mAU(AU = Anson unit). The Novagen ProteinaseK products are free of detectable DNase andRNase
Application
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Biochem/physiol Actions
Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.
Other Notes
One AU (AU = Anson unit) is defined as the amount of enzyme that liberates 1.0 µmol (181 µg) of tyrosine from casein per minute at pH 7.5 at 37°C.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)
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This Item | |||
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| description Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA. | description free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥30 units/mg protein | description Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥30 units/mg protein, Molecular Biology | description Proteinase K Inhibitor is a tetrapeptidyl chloromethyl ketone compound that acts as an active-site-targeting irreversible inhibitor against proteinase K. |
| technique(s) DNA extraction: suitable | technique(s) - | technique(s) RNA extraction: suitable, DNA extraction: suitable | technique(s) - |
| form solid | form - | form lyophilized powder | form lyophilized |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in ambient |
| mol wt 28.93 kDa | mol wt 28.93 kDa | mol wt 28.93 kDa, 28,930 Da | mol wt - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| storage condition OK to freeze | storage condition - | storage condition dry at room temperature | storage condition OK to freeze, protect from light, desiccated |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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After freeze proteinase K 70663 and it warms to room temp. How long is that good ?
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