604980

Thrombin, Bovine, High Activity

• Synonym(s): Thrombin, Bovine, High Activity
• CAS Number: 9002-04-4
• MDL number: MFCD20921901
• NACRES: NA.25
• UNSPSC Code: 12352204
• Specific activity: ≥1800 NIH units/mg protein
• Biological source: bovine

Properties

• biological source: bovine
• Quality Segment: 100
• description: Merck USA index - 14, 9383
• form: lyophilized
• specific activity: ≥1800 NIH units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• solubility: water: soluble
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Thrombin is a sodium-activated type II enzyme. This serine protease enzyme consists of two anion binding exosites, ABE-I and ABE-II.[1] Thrombin is synthesized from zymogen prothrombin (factor II) in the liver. Bovine A-thrombin comprises a light chain (A chain) and a heavy chain (B chain). These two chains are linked by non-covalent interactions and by one disulfide bond.[2]

Application

Thrombin, bovine, high activity has been used:

• to stimulate LNCaP cell and to study the effect of activation of G-protein-coupled receptors (GPCRs)[3]
• in fibrin gel fabrication[4]
• to form fibrinogen gel by fibrinogen gel-based method[4]

Biochem/physiol Actions

Thrombin converts fibrinogen into fibrin and activates factors V, VIII, XI, and XIII.[5] It promotes platelet activation and vascular contraction. Thrombin stabilizes the fibrin polymers.[2] It plays a crucial role in the final stages of the blood coagulation cascade.[1]

Physical form

Lyophilized from 200 mM NaCl, 50 mM sodium citrate, 1 ml 0.1% PEG, pH 6.5.

Analysis Note

Complete activation from homogeneous prothrombin by SDS-PAGE

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable