This product has a concentration of 50 µg/mL. To obtain a 7.5 mg equivalent, 150 mL of this product is required. Since Vindelov's PI is typically provided with propidium iodide at a concentration of 75 µg/mL, using this less concentrated product is not ideal for preparation. Please see the link below to review P4864, a 1 mg/mL PI solution that may be of interest:
https://www.sigmaaldrich.com/product/sial/p4864
537060
Propidium Iodide Solution
A convenient form of propidium iodide useful for flow cytometry studies.
Synonym(s):
Propidium Iodide Solution
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About This Item
Empirical Formula (Hill Notation):
C27H34I2N4
CAS Number:
Molecular Weight:
668.39
UNSPSC Code:
12352200
NACRES:
NA.47
MDL number:
Form:
liquid
Storage condition:
OK to freeze, protect from light
Pricing and availability is not currently available.
Quality Segment
description
RTECS - SF7949600
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
input
sample type intact cells
fluorescence
λex 536 nm, λem 617 nm
shipped in
ambient
storage temp.
2-8°C
SMILES string
[I-].I.[N+](CCCN1C2=CC(=N)C=CC2=C3C(=C1c4ccccc4)C=C(C=C3)N)(CC)(CC)C
InChI
1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChI key
XJMOSONTPMZWPB-UHFFFAOYSA-M
General description
Membrane impermeant DNA intercalator. Useful for staining apoptotic cells and nuclei by flow cytometry. Useful in differentiating between apoptotic and necrotic cell death. Can be used together with common green fluorescent probes, e.g. fluorescein isothiocyanate. Excitation max.: ~536 nm; Emission max.: ~617 nm.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Membrane impermeable DNA intercalator
Membrane impermeable DNA intercalator
Product does not compete with ATP.
Reversible: no
Physical form
50 µg/ml in PBS, pH 7.4.
Preparation Note
Following initial use, aliquot and freeze (-20°C).
Other Notes
Belloc, F., et al. 1994. Cytometry17, 59.
Cropmton, T., et al. 1992. Biochem. Biophys. Res. Commun.183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.
Cropmton, T., et al. 1992. Biochem. Biophys. Res. Commun.183, 532.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
de Caestecker, M.P., et al. 1992. J. Immunol. Methods154, 11.
Pollice, A.A., et al. 1992. Cytometry13, 432.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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This Item | |||
|---|---|---|---|
| description A convenient form of propidium iodide useful for flow cytometry studies. | description ≥94% (HPLC) | description - | description Membrane impermeable DNA intercalator. |
| form liquid | form powder | form liquid | form solid |
| manufacturer/tradename Calbiochem® | manufacturer/tradename - | manufacturer/tradename - | manufacturer/tradename Calbiochem® |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| storage condition OK to freeze, protect from light | storage condition - | storage condition - | storage condition OK to freeze, protect from light |
| shipped in ambient | shipped in - | shipped in - | shipped in ambient |
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Storage Class
12 - Non Combustible Liquids
WGK
WGK 1
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I already have this product. How many mL should be used to get a 7.5mg equivalent amount for Vindelov's PI?
1 answer-
Helpful?
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what is the concentration of the PI solution
1 answer-
The concentration is 50 µg/ml in PBS, pH 7.4.
Helpful?
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What is the recommended protocol for use/ working concentration?
1 answer-
The working concentration must be determined by the end user.
In the following reference: Cytometry. 1994 Sep 1;17(1):59-65. PMID: 7528124 DOI: 10.1002/cyto.990170108; 5ug/ML of PI was directly added in the culture medium for 30min. at 37C.Helpful?
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