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524653

PBS-TWEEN® Tablets

tablet, 140 mM NaCl, 3 mM KCl, 0.05% TWEEN 20 Detergent, 10 mM phosphate buffer, pH 7.4 at 25°C

Synonym(s):

PBS, Phosphate Buffered Saline-TWEEN® 20 Tablets

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About This Item

UNSPSC Code:
41105319
NACRES:
NA.25
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Product Name

PBS-TWEEN® Tablets, Dissolve one tablet in 1 liter of deionized water to yield 140 mM NaCl, 3 mM KCl, 0.05% TWEEN 20 Detergent, 10 mM phosphate buffer, pH 7.4 at 25°C. Note: 1 each = 10 tablets.

Quality Segment

form

tablet

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

solubility

deionized water: 1 tablet/liter

shipped in

ambient

storage temp.

15-25°C

General description

Developed for use in immunoassay applications. Dissolving one tablet in 1 liter of deionized H2O yields 140 mM NaCl, 2.7 mM KCl, 0.05% TWEEN® 20 detergent, and 10 mM phosphate buffer, pH 7.4 at 25°C. Note: 1 each = 10 tablets.
Dissolve one tablet in 1 liter of deionized water to yield 140 mM NaCl, 3 mM KCl, 0.05% TWEEN® 20 Detergent, 10 mM phosphate buffer, pH 7.4 at 25°C. Note: 1 each = 10 tablets.

Application

PBS-TWEEN® Tablets have been used as a:
  • diluting agent in immunohistochemical staining
  • wash buffer during enzyme-linked immunosorbent assay (ELISA)
  • blocking agent during Western blotting

Preparation Note

Following reconstitution, store in the refrigerator (4°C) or filter sterilized and store at room temperature (20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Disclaimer

Toxicity: Irritant (B)

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This Item
524753N2770S8820
description

Dissolve one tablet in 1 liter of deionized water to yield 140 mM NaCl, 3 mM KCl, 0.05% TWEEN 20 Detergent, 10 mM phosphate buffer, pH 7.4 at 25°C. Note: 1 each = 10 tablets.

description

Dissolving one tablet in 500 ml of deionized water yields 150 mM NaCl, 0.05% TWEEN 20 Detergent, 50 mM Tris-HCl buffer, pH 7.6 at 25°C. Supplied in convenient blister packs. Note: 1 each = 10 tablets.

description

tablet, To prepare 20 mL

description

For General Use

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

200

form

tablet

form

-

form

tablet

form

tablet

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

-

storage condition

-

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

−20°C

storage temp.

2-8°C

solubility

deionized water: 1 tablet/liter

solubility

-

solubility

water: 0.25 tablet/mL, clear, colorless

solubility

water: 1 tablet/100mL, clear to hazy, colorless

shipped in

ambient

shipped in

ambient

shipped in

-

shipped in

-


Storage Class

11 - Combustible Solids

wgk

WGK 3



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Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. Is this product sterile/usable in the context of microbiological analysis ?

    1 answer
    1. The product is not sterile. No protocol is offered concerning its use in microbiological analyses.

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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