374825

Hemocyanin, Keyhole Limpet, Megathura crenulata, High Purity, Endotoxin-Free, Sterile-Liquid

Hemocyanin, Keyhole Limpet, Megathura crenulata, High Purity, Endotoxin Free, is a large, multi-subunit, O2-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.

• Synonym(s): Hemocyanin, Keyhole Limpet, Megathura crenulata, High Purity, Endotoxin-Free, Sterile-Liquid
• CAS Number: 9013-72-3
• UNSPSC Code: 12352202
• NACRES: NA.25
• MDL number: MFCD00131280

Properties

• Quality Segment: 200
• assay: ≥95% (size exclusion chromatography)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• impurities: ≤25 EU/mg Endotoxin, ≥1.5 x 10⁻³ copper to protein ratio, ≥5 mg/mL Protein
• color: clear bluish
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Keyhole limpet hemocyanin (KLH) is a large, multi-subunit, O2-carrying, metalloprotein. This high molecular weight glycoprotein is extracted from a marine snail species called Megathura crenulate.

Application

Hemocyanin, Keyhole Limpet, Megathura crenulata, high purity, endotoxin-free, sterile-liquid has been used as an antigen to test the polyreactivity of monoclonal antibody (mAb) using enzyme-linked immunosorbent assay(ELISA). It has also been used to immunize animals to study KLH-specific ex vivo recall responses and specific T-cell dependent antibody response (TDAR) in monkeys.

Biochem/physiol Actions

Keyhole limpet hemocyanin (KLH) stimulates both cell-mediated and humoral responses in animals and humans. It elicits immunostimulatory properties and also functions as a vaccine adjuvant. KLH serves as a carrier protein to produce antibodies and haptens like peptides and oligosaccharides. It is used to treat bladder cancer.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 83 mM sodium phosphate, 150 mM NaCl, pH 7.2.

Preparation Note

KLH is an extremely large protein complex that has a tendency to aggregate and precipitate from solutions. Use a buffer when diluting KLH and maintain the magnesium concentration at ≥10 mM. Store KLH solutions at 4°C. DO NOT freeze, vortex or agitate to dissolve KLH. Freezing or agitating KLH will cause protein aggregation and precipitation.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 2