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Calpain-1 Substrate, Fluorogenic

Name: Calpain-1 Substrate, Fluorogenic
Brand: MM

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin.

Synonym(s):

Calpain-1 Substrate, Fluorogenic, μ-Calpain Substrate, Fluorogenic, H-K(FAM)-EVY~GMMK(DABCYL)-OH

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About This Item

Empirical Formula (Hill Notation):

C₇₉H₉₅N₁₃O₁₉S₂

Molecular Weight:

1594.81

NACRES:

NA.77

UNSPSC Code:

12352200

Assay:

≥95% (HPLC)

Form:

lyophilized solid

Storage condition:

OK to freeze, desiccated (hygroscopic), protect from light

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Properties

Quality Segment

100

assay

≥95% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, desiccated (hygroscopic), protect from light

color

red

solubility

DMSO: 1 mg/mL

fluorescence

λ_ex ~490 nm, λ_em ~518 nm

shipped in

wet ice

storage temp.

−20°C

Description

General description

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (K_m = 4.6 µM; k_cat = 11 s^-1). Cleavage occurs at the Tyr-Gly bond and results in enhanced fluorescence. Excitation max: ~490 nm; emission max: ~518 nm.

Biochem/physiol Actions

Cell permeable: no

Primary Target
A sensitive and specific substrate for calpain-1

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Physical form

Supplied as a trifluoroacetate salt.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

H-Lys(FAM)-Glu-Val-Tyr~Gly-Met-Met-Lys(DABCYL)-OH

Mittoo, S., et al. 2003. Anal. Biochem.319, 234.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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This Item	208771	208731	218739
Sigma-Aldrich 208748 Calpain-1 Substrate, Fluorogenic	Sigma-Aldrich 208771 Calpain Substrate III, Fluorogenic	Sigma-Aldrich 208731 Calpain Substrate II, Fluorogenic	Sigma-Aldrich 218739 Caspase-1 Substrate X, Fluorogenic
description An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin.	description A fluorogenic FRET peptide with a substrate sequence that is optimized for calpain-1 and -2.	description Cyclic fluorogenic substrate for the quantitative determination of calpain -1 and -2.	description A fluorogenic substrate for detecting caspase-1 (ICE), caspase-4, and caspase-5 activity.
form lyophilized solid	form lyophilized solid	form solid	form lyophilized
assay ≥95% (HPLC)	assay ≥98% (HPLC)	assay ≥95% (TLC)	assay ≥95% (HPLC)
manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
solubility DMSO: 1 mg/mL	solubility DMSO: 5 mg/mL, water: 1 mg/mL	solubility DMSO: soluble	solubility DMSO: 5 mg/mL

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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