The Histone H3 protein in 14-494 is full length human Histone H3, the sequence of which should be the same as NP_003520.1, without any affinity tags. Its methylation status is not determined. On the other hand, Catalog # 12-563 is Monomethyl-Histone H3 (Lys4) Peptide, biotin conjugate. This product is a synthetic peptide, corresponding to amino acids 1-21 of Histone H3 monomethylated at Lys4 followed by a GG linker and biotinylated lysine [ART(mmK)QTARKSTGGKAPRKQLA -GGK-biotin].
14-494
Histone H3, human recombinant
Recombinant human histone H3.
Synonym(s):
H3, Histone H3
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
eCl@ss:
32160405
Biological source:
human
Recombinant:
expressed in E. coli
Mol wt:
Mw ~17 kDa
Pricing and availability is not currently available.
biological source
human
Quality Segment
recombinant
expressed in E. coli
mol wt
Mw ~17 kDa
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... H3F3B(3021)
General description
Product Source: human histone H3 produced in E. coli
Application
Recombinant human histone H3.
Packaging
also available in larger sizes, please inquire for pricing and availability.
Physical form
FPLC agarose column
Lyophilized powder
Preparation Note
Lyophilized: Stable for 2 years at -20°C from date of shipment. Rehydrated: Stable for 6 months at -20°C. Aliquot rehydrated solution to avoid repeated freezing and thawing
Analysis Note
routinely evaluated by its use as a substrate for in vitro enzymatic reactions, such as acetylation by PCAF
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Hi, I need H3 with a methyl group on lysine 4, which is H3(K4(me1). Could you please let me know what the H3 sequence is in 14-494? Is there any tag with H3? Is there a methyl group on lysine 4 (K4(me1))? Thank you in advance Tooba Hallaj
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