Skip to Content
Merck
All Photos(1)

Key Documents

1.25062

Millipore

Fractogel® EMD COO- (M)

MiniChrom Fractogel® COO (M), volume 1 mL, prepacked column

Synonym(s):

MiniChrom Column Fractogel® COO (M), 1 ml

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41115711

ligand

(Carboxyethyl )

material

polypropylene housing

product line

Fractogel®

form

prepacked column

parameter

≤8 bar pressure
~300 cm/hr max. flow rate

column I.D. × L

8 mm × 20 mm

volume

1 mL

matrix active group

Methacrylate

mean particle size

40-90 μm

capacity

~100 mL, resin binding capacity

application(s)

mAb
recombinant protein
vaccine development

separation technique

cation exchange

storage temp.

15-25°C

General description

8 x 20mm

Features and Benefits

MiniChrom Column Fractogel® COO (M), 1 ml enables:
  • Good resolution between monomers and aggregates
  • Efficient aggregate and HCP removal combined with high yield
  • Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution


Due to the titration behavior, the ion exchange capacity can be used from pH 6 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel® EMD COO- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:
  • the buffer system
  • pH value of the buffer which determines the surface charge of the protein
  • the degree of the ionization of the functional groups of the exchanger
  • the concentration of the counter ions
  • the charge density on the support (protein binding capacity)

Analysis Note

Appearance: passes test

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service