108975

Leupeptin, Hemisulfate, Microbial

Leupeptin hemisulfate, CAS 103476-89-7, is a reversible inhibitor of trypsin-like proteases and cysteine proteases

• Synonym(s): Leupeptin, Hemisulfate, Microbial, Ac-LLR-CHO, ½H₂SO₄
• Empirical Formula (Hill Notation): C₂₀H₃₈N₆O₄ · 0.5H₂SO₄
• CAS Number: 103476-89-7
• Molecular Weight: 475.59
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00037012
• Assay: ≥90% (HPLC)
• Form: lyophilized solid
• Storage condition: OK to freeze, desiccated (hygroscopic)

Properties

• Quality Segment: 200
• description: Merck USA index - 14, 5456
• assay: ≥90% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated (hygroscopic)
• color: white
• solubility: water: 50 mg/mL
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: [N+H](=C(N)N)CCC[C@H](NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)C)CC(C)C)CC(C)C)C=O
• InChI: 1S/C20H38N6O4/c1-12(2)9-16(24-14(5)28)19(30)26-17(10-13(3)4)18(29)25-15(11-27)7-6-8-23-20(21)22/h11-13,15-17H,6-10H2,1-5H3,(H,24,28)(H,25,29)(H,26,30)(H4,21,22,23)/p+1/t15-,16-,17-/m0/s1
• InChI key: GDBQQVLCIARPGH-ULQDDVLXSA-O

Description

General description

A reversible inhibitor of trypsin-like proteases and cysteine proteases. Also known to inhibit activation-induced programmed cell death and to restore defective immune response in HIV⁺ donors. Often used for the inhibition of endoproteinase Lys-C, kallikrein, papain, plasmin, thrombin, cathepsin B, and trypsin. Leupeptin does not inhibit chymotrypsin, elastase, renin, or pepsin. The recommended working range is 10-100 µM for most applications.

Biochem/physiol Actions

Cell permeable: no

Primary Target
trypsin- and cysteine-like proteases

Product does not compete with ATP.

Reversible: yes

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Aqueous stock solutions are stable for up to 1 week at 4°C or for up to 1 month at -20°C.

Other Notes

Ac-Leu-Leu-arginal, hemisulfate

Montenez, J.P., et al. 1994. Toxicol. Lett. 73, 201.
Sarin, A., et al. 1994. J. Immunol.153, 862.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable