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368881

Sigma-Aldrich

Fast Red TR Salt

Dye content 15 %

Synonym(s):

Fast Red TR Salt hemi(zinc chloride) salt, 4-Chloro-2-methylbenzenediazonium salt, Azoic Diazo No. 11

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About This Item

Empirical Formula (Hill Notation):
C7H6Cl2N2 · 0.5 ZnCl2
CAS Number:
89453-69-0
Molecular Weight:
257.19
Colour Index Number:
37085
MDL number:
MFCD00074766
UNSPSC Code:
12171500
PubChem Substance ID:
24862940
NACRES:
NA.47
Pricing and availability is not currently available.

form

powder

Quality Level

200

composition

Dye content, 15%

λmax

284 nm

ε (extinction coefficient)

≥3000 at 281-287 nm in water at 0.006 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].Cl[Zn]Cl.Cc1cc(Cl)ccc1[N+]#N.Cc2cc(Cl)ccc2[N+]#N

InChI

1S/2C7H6ClN2.4ClH.Zn/c2*1-5-4-6(8)2-3-7(5)10-9;;;;;/h2*2-4H,1H3;4*1H;/q2*+1;;;;;+2/p-4

InChI key

RIIVFDKBXXMYRL-UHFFFAOYSA-J

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General description

Fast Red TR is a diazonium dye. It serves as a chromogenic substrate for alkaline phosphatase (AP), a common antibody conjugate widely used fin immunohistochemical applications.

Application

Fast Red TR Salt hemi (zinc chloride) salt has been used in the preparation of staining solutions to stain TRACP (tartrate-resistant acid phosphatase). It is also used for the detection of alkaline phosphatase.

Pictograms

CorrosionEnvironment
GHS05,GHS09

Signal Word

Danger

Hazard Statements

H314,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1C

Storage Class Code

8A - Combustible corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SHBR6181
MKCJ2482
MKBF9253
24123JB
15610BR

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Kessler C
Nonradioactive Labeling and Detection of Biomolecules (2012)
Effect of monoterpenes on the formation and activation of osteoclasts in vitro
Dolder S
Journal of Bone and Mineral Research, 21(4), 647-655 (2006)
Reliability of new poly (lactic-co-glycolic acid) membranes treated with oxygen plasma plus silicon dioxide layers for pre-prosthetic guided bone regeneration processes
Castillo-Dali G
Medicina Oral, patologia Oral y cirugia Bucal, 22(2), e242-e250 (2017)
The effects of 4-chloro-ortho-toluidine (fast red TR) subacute intoxication on rats. Part I: The kidney and the urinary bladder epithelium.
A Godlewski et al.
Folia morphologica, 55(4), 253-255 (1996-01-01)
T Kenzaka et al.
Microbiology (Reading, England), 144 ( Pt 8), 2085-2093 (1998-08-28)
An improved in situ hybridization technique, HNPP-FISH, using 2-hydroxy-3-naphthoic acid 2'-phenylanilide phosphate (HNPP) and Fast Red TR was applied to analyse the community structure of planktonic bacteria in river water. Oligonucleotide probes specific for the domain Bacteria (EUB338) and five
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