Monoclonal Anti-TGN46 (mouse IgG1 isotype) is derived from the hybridoma TGN46-52 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human TGN46 conjugated to KLH. Trans-Golgi network protein 46-kDa (TGN46), It is a heavily glycosylated protein, containing a signal peptide, a lumenal domain, a membrane-spanning domain and a cytoplasmic domain. The membrane spanning region and the cytoplasmic tail contain the retention and retrieval signals for localization in the TGN.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper)
Monoclonal Anti-TGN46 antibody produced in mouse has been used in immunoblotting and immunoprecipitation.
Biochem/physiol Actions
Trans-Golgi network protein, 46-kDa (TGN46) is involved in regulating membrane traffic to and from the TGN. It cycles constitutively between the TGN and the plasma membrane, returning via endosomes.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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Essential biological systems employ self-correcting mechanisms to maintain cellular homeostasis. Mammalian cell function is dynamically regulated by the interaction of cell surface galectins with branched N-glycans. Here we report that N-glycan branching deficiency triggers the Golgi to generate bioequivalent N-glycans
Wnt signaling through the Frizzled (FZD) family of serpentine receptors is essential for embryogenesis and homeostasis, and stringent control of the FZD protein level is critical for stem cell regulation. Through CRISPR/Cas9 genome-wide screening in human cells, we identified TMEM79/MATTRIN
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