L6512

Lectin from Helix pomatia

biotin conjugate, lyophilized powder

• Synonym(s): Helix pomatia agglutinin, HPA
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• conjugate: biotin conjugate
• Quality Level: 200
• form: lyophilized powder
• extent of labeling: 2-4 mol biotin per mol protein
• impurities: salt, free
• storage temp.: −20°C

Description

General description

Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals.[1] These oligomeric proteins are characterized by the presence of a carbohydrate recognition domain.[2]

Application

Lectin from Helix pomatia has been used to study its binding activities in Gyrodactylus derjavini parasitizing fins of Oncorhynchus mykiss.[3]

Biochem/physiol Actions

HPA has anti-A human blood group specificity and has an affinity for terminal N-acetyl-α-D-galactosaminyl residues. Conjugates are prepared from affinity purified lectin.

Lectin is useful in glycoconjugate characterizing, imaging and targeting. Lectin participates in host recognition and tissue adhesion.[1] Lectins shows resistance to proteolytic degradation in vivo.[4]

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hour incubation at 25 °C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves