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B4679

Sigma-Aldrich

BIS-TRIS propane

BioPerformance Certified, suitable for cell culture, ≥99.0%

Synonym(s):

1,3-Bis[tris(hydroxymethyl)methylamino]propane

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About This Item

Linear Formula:
CH2[CH2NHC(CH2OH)3]2
CAS Number:
64431-96-5
Molecular Weight:
282.33
Beilstein:
1786109
EC Number:
264-899-3
MDL number:
MFCD00004689
UNSPSC Code:
12161700
PubChem Substance ID:
24891799
NACRES:
NA.25

grade

BioPerformance Certified

Assay

≥99.0%

form

crystals

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin and bioburden, tested

useful pH range

6.3-9.5

pKa (25 °C)

(1) 6.8, (2) 9.0

mp

164-165 °C (lit.)

application(s)

diagnostic assay manufacturing

foreign activity

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

SMILES string

OCC(CO)(CO)NCCCNC(CO)(CO)CO

InChI

1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2

InChI key

HHKZCCWKTZRCCL-UHFFFAOYSA-N

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Application

  • Neutralized chimeric DNA probe for the improvement of GC-rich RNA detection specificity on the nanowire field-effect transistor.: BIS-TRIS propane is used in the development of nanowire field-effect transistors for detecting GC-rich RNA sequences. The study demonstrates the buffer′s role in enhancing the specificity and sensitivity of these biosensors (Chou et al., 2019).

  • Elimination of ghost peaks by optimization of anion exchange chromatography method for determination of gamma-carboxyglutamic acid (Gla)-domainless impurity in recombinant activated clotting factor VII drug products.: BIS-TRIS propane is employed in optimizing chromatography methods to eliminate ghost peaks during the analysis of pharmaceutical impurities. The buffer′s effectiveness ensures accurate and reliable detection of impurities in drug formulations (Tahmasbi et al., 2019).

Other Notes

Easily compare specifications for BIS-TRIS propane products with the BIS-TRIS propane specification table.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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R Tóth-Boconádi et al.
Biophysical journal, 78(6), 3170-3177 (2000-05-29)
Buffers change the electric signals of light-excited bacteriorhodopsin molecules in purple membrane if their concentration and the pH of the low-salt solution are properly selected. "Positive" buffers produce a positive component, and "negative" buffers a negative component in addition to
R Coronado et al.
The Journal of general physiology, 79(4), 529-547 (1982-04-01)
A collection of organic cations has been used to probe the gross structural features of the ionic diffusion pathway in a K+-selective channel from sarcoplasmic reticulum (SR). Channels were incorporated into planar phospholipid bilayer membranes, and single-channel currents were measured
Sami J O Varjo et al.
Journal of chromatography. A, 1081(1), 92-98 (2005-07-15)
Greater stability of liposome coatings and improved resolution of model steroids in capillary electrochromatography (CEC) were sought by adding small diamines (ethylenediamine, diaminopropane, bis-tris-propane, or N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid, HEPES)) to the liposome solution before coating of fused silica capillaries. The phospholipid
P Gómez-Tagle et al.
Inorganic chemistry, 40(15), 3786-3796 (2001-07-10)
Potentiometric titrations of the mixtures of lanthanide(III) perchlorates and bis-Tris propane (BTP) reveal formation of dinuclear hydroxo complexes M2(BTP)2(OH)n(6-n), where M = La(III), Pr(III), Nd(III), Eu(III), Gd(III), and Dy(III) and n = 2, 4, 5, or 6, in the pH
Joseph S Brunzelle et al.
Archives of biochemistry and biophysics, 474(1), 157-166 (2008-04-01)
The three-dimensional structure of the catalytically efficient beta-xylosidase from Selenomonas ruminantium in complex with competitive inhibitor 1,3-bis[tris(hydroxymethyl)methylamino]propane (BTP) was determined by using X-ray crystallography (1.3A resolution). Most H bonds between inhibitor and protein occur within subsite -1, including one between
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