Cytotoxicity LDH Assay Kit-WST is a kit for determination of cytotoxicity by measuring a lactate dehydrogenase (LDH) activity released from damaged cells. LDH is a stable cytoplasmic enzyme present in all types of cells and released into the cell culture medium through damaged plasma membrane. Since Cytotoxicity LDH Assay kit-WST neither reflects the activity of living cells nor is harmful to cells, cytotoxicity can be measured with living cells (homogeneous assay). In addition, it is possible to perform a non-homogeneous assay by using the cell culture supernatant.
After preparation the Working Solution is stable under refrigerated conditions and can readily be used for up to six months.
Features and Benefits
Colorimetric assay system
Utilize for both homo- and non-homogeneous assays
Can be used with and without transferring supernatant
The excessive and inappropriate production of reactive oxygen species (ROS) can cause oxidative stress and is implicated in the pathogenesis of lung cancer. Cyclophilin A (CypA), a member of the immunophilin family, is secreted in response to ROS. To determine
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