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83200

Sigma-Aldrich

Pyronin Y

for microscopy (Bot., Fl., Hist.)

Synonym(s):

Pyronin G, Pyronin J

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About This Item

Empirical Formula (Hill Notation):
C17H19ClN2O
CAS Number:
92-32-0
Molecular Weight:
302.80
Colour Index Number:
45005
Beilstein:
3795789
EC Number:
202-147-8
MDL number:
MFCD00011725
UNSPSC Code:
12171500
PubChem Substance ID:
57652949
NACRES:
NA.47

grade

for microscopy (Bot., Fl., Hist.)

Quality Level

100

mp

250-260 °C (lit.)

solubility

water: 0.01 g/10 mL, red

εmax

≥1650 at 543-553 nm in 50% ethanol
≥650 at 249-259 nm in 50% ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C

InChI

1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1

InChI key

INCIMLINXXICKS-UHFFFAOYSA-M

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Biochem/physiol Actions

Pyronin Y is important in histochemistry and is combined with methyl green for staining of RNA. The mixture results in bright red/green staining of nucleic acids.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Rost FWD
Fluorescence Microscopy (1995)
Karsten Kuritz et al.
Scientific reports, 10(1), 3619-3619 (2020-02-29)
Modern cytometry methods allow collecting complex, multi-dimensional data sets from heterogeneous cell populations at single-cell resolution. While methods exist to describe the progression and order of cellular processes from snapshots of such populations, these descriptions are limited to arbitrary pseudotime
S V Desai et al.
International endodontic journal, 44(10), 907-916 (2011-05-14)
To investigate the expression of TLR2 in refractory periapical lesions. Refractory periapical lesion biopsies were histopathologically and clinically categorized into asymptomatic periapical granuloma (n=10), symptomatic periapical granuloma (n=10) or periapical cyst (n=10) and prepared for immunohistochemical staining using antibodies to
Pedro Montes-Navajas et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 8(12), 1743-1747 (2009-12-22)
Cucurbiturils (CBs) of the appropriate size (CB[7] and CB[8]) form strong guest-host complexes in phosphate buffer solution (PBS) with acridine orange (AO) and pyronine Y (PYY) with 1 : 1 and 2 : 1 stoichiometries for CB[7] and CB[8] complexes
Yuichiro Koide et al.
ACS chemical biology, 6(6), 600-608 (2011-03-08)
The absorption and emission wavelengths of group 14 pyronines and rhodamines, which contain silicon, germanium, or tin at the 10 position of the xanthene chromophore, showed large bathochromic shifts compared to the original rhodamines, owing to stabilization of the LUMO
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