MAB3422
Anti-Histone Antibody, clone H11-4
clone H11-4, Chemicon®, from mouse
Synonym(s):
Anti-Histone antibody
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
H11-4, monoclonal
species reactivity
hamster, bovine, opossum, rat, human, Xenopus
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... H2AC21(317772)
General description
There are 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. The five major classes of histones are H1/H5, H2A, H2B, H3, and H4. Histones H2A, H2B, H3 and H4 are known as the core histones, while histones H1 and H5 are known as the linker histones. Histones feature a main globular domain and a long N terminal tail which are involved with the structure of the nucleosomes of the ′beads on a string′ structure. They are the chief protein components of chromatin, acting as spools around which DNA winds, and play a role in gene regulation.
Specificity
Due to the highly conserved nature of histones, this antibody is predicted to have wide species reactivity.
The antibody binds specifically to an antigen determinant, which is present on all five histone proteins. The antibody recognizes the histones H1, H2A, H2B, H3 and H4 with comparable sensitivity.
Immunogen
Purified nuclear fractions of HeLa cells
Application
Detect Histone with Anti-Histone Antibody, clone H11-4 (Mouse Monoclonal Antibody), that has been shown to work in ICC, IHC & WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Western Blot Analysis:
1:500 dilution of a previous lot was used.
Immunocytochemistry:
A 1-5 µg/mL concentration of a previous lot was used on Immunocytochemistry.
Immunohistochemistry:
A 1-5 μg/mL concentration of a previous lot was used on Immunohistochemistry.
Optimal working dilutions must be determined by end user.
1:500 dilution of a previous lot was used.
Immunocytochemistry:
A 1-5 µg/mL concentration of a previous lot was used on Immunocytochemistry.
Immunohistochemistry:
A 1-5 μg/mL concentration of a previous lot was used on Immunohistochemistry.
Optimal working dilutions must be determined by end user.
Quality
Routinely evaluated by Western Blot on Jurkat lysates.
Physical form
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing 0.02M phosphate buffer, pH 7.6 with 0.25M NaCl and 0.1% sodium azide
Storage and Stability
Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
The Iyophilized antibody is stable at 2–8°C. Store the reconstituted antibody in aliquots at -15 to -25°C for up to 12 months.Repeated freezing and thawing should be avoided.
The Iyophilized antibody is stable at 2–8°C. Store the reconstituted antibody in aliquots at -15 to -25°C for up to 12 months.Repeated freezing and thawing should be avoided.
Analysis Note
Control
All tissue
All tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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