IM33

Anti-MMP-2 (Ab-3) Mouse mAb (42-5D11)

liquid, clone 42-5D11, Calbiochem^®

• Synonym(s): Anti-72 kDa Gelatinase, Anti-Gelatinase A, Anti-Matrix Metalloproteinase 2
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 42-5D11, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: bovine, rat, mouse, human
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MMP2(4313)

Description

General description

Purified mouse monoclonal antibody (see application references). Recognizes the ~72 kDa latent and the ~66 kDa active forms of MMP-2.

Recognizes the ~72 kDa latent and the ~66 kDa active forms of the MMP-2.

This Anti-MMP-2 (Ab-3) Mouse mAb (42-5D11) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of MMP-2 (Ab-3).

Immunogen

Epitope: within amino acids 468-483 of human MMP-2 (numbered from the propeptide)

Human

a synthetic peptide (VTPRDKPMGPLLVATF) corresponding to amino acids 468-483 of human MMP-2

Application

Frozen Sections (see application references)
Immunoblotting (1 g/ml)
Paraffin Sections (see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
MMP-9 protein (Cat. Nos. PF024 or PF038)

Positive Control
MMP-2 protein (Cat. Nos. PF023 or PF037)

Other Notes

Cottam, D. W. and Rees, R. C. 1993. Intl J. Oncol.2, 861.
Fujimoto, N., et al. 1993. Clinica Chimica Acta.221, 91.
Stetler-Stevenson, W. G., et al. 1993. FASEB J.7, 1434.
Woessner, J. F. 1991. FASEB J.5, 2145.
Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 99.

This antibody will not cross-react with human MMP-1, MMP-3, MMP-9, or MMP-13. Antibody should be titrated for optimal results in individual systems.

Legal Information

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable