05-516-AF555
Anti-Ras Antibody, clone RAS10, Alexa Fluor™ 555 Conjugate
clone RAS10, from mouse, ALEXA FLUOR™ 555
Synonym(s):
GTPase HRas, c-H-ras, H-Ras-1, Ha-Ras, p21ras, Transforming protein p21, GTPase KRas, c-K-ras, c-Ki-ras, K-Ras 2, Ki-Ras, GTPase NRas, Transforming protein N-Ras
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
Recommended Products
biological source
mouse
Quality Level
conjugate
ALEXA FLUOR™ 555
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
RAS10, monoclonal
species reactivity
rat, human, mouse
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... HRAS(3265) , KRAS(3845) , NRAS(4893)
General description
Ras proteins are small GTPases controlling cellular pathways that signal for growth, proliferation, and differentiation. Mammalian genomes harbor three Ras genes (HRAS, NRAS, and KRAS) that encode four GTPases, two of which (K-Ras4A and K-Ras4B) are transcribed from the KRAS gene via two alternative fourth exons. Ras proteins share nearly identical sequence homology in sequence encoded by exons 1-3 (a.a. 1-165), while diverge in their C-terminal 24-a.a. hypervariable region (HVR) encoded by exon 4. The first 165 amino acids form the catalytic G domain that mediates GDP/GTP binding and association with effectors, exchange factors, and GTPase-activating proteins (GAPs), while the C-terminal HVR is needed for Ras membrane localization, a process necessary for Ras function. In addition to the removal of initiator methionine (Met1), Ras proteins are posttranslationally modified by farnesylation of Cys186, followed by proteolytical cleavage their C-terminal end three amino acids (a.a. 187-189) and carboxyl methylation of the newly formed C-terminal prenylcysteine. H-Ras, N-Ras, and K-Ras4A are further palmitoylated on cysteine residue(s) near the C-terminus (Cys181/Cys184 in H-Ras, Cys181 in N- and K-Ras), while K-Ras4B lacks a palmitoylation site. Palmitoylation is required for targeting H- and N-Ras to the plasma membrane (PM), while K-Ras4A and K-Ras4B contain a series of lysines that constitute a polybasic region (PBR) capable of interacting with the inner leaflet of the PM through an electrostatic interaction.
Specificity
Ras 10 reacts with normal p21, activated p21, and p21 from each of the Harvey, Kirsten and N-ras families. Ras 10 reacts with recombinant p21 as well as p21 derived from human and rodent cells (Hamer, P.J., et al. (1990). Hybridoma.9(6):573-587).
Immunogen
Recombinant human H-Ras p21 (Hamer, P.J., et al. (1990). Hybridoma.9(6):573-587).
Application
Detect Ras using this mouse monoclonal Anti-Ras Antibody, clone RAS10, Alexa Fluor™ 555 Conjugate, Cat. No. 05-516-AF555, validated for use in Flow Cytometry aned Immunocytochemistry.
Flow Cytometry Analysis: 0.2 µL from a representative lot detected Ras immunoreactivity in one million A431 cells.
Clone Ras10 is also available as unconjugated, as well as in FITC- and Alexa Fluor™ 647-conjugated forms (Cat. Nos. 05-516, FCMAB148F, 05-516-AF647) for ELISA, flow cytometry, immunocytochemistry, immunohistochemistry, immunoprecipitation, and Western blotting applications.
Clone Ras10 is also available as unconjugated, as well as in FITC- and Alexa Fluor™ 647-conjugated forms (Cat. Nos. 05-516, FCMAB148F, 05-516-AF647) for ELISA, flow cytometry, immunocytochemistry, immunohistochemistry, immunoprecipitation, and Western blotting applications.
Research Category
Signaling
Signaling
Quality
Evaluated by Immunocytochemistry in A431 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Ras immunoreactivity in A431 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Ras immunoreactivity in A431 cells.
Target description
~21 kDa observed. 21.30 kDa (human/mouse/rat H-Ras4A, p21 pro-form), 20.87 kDa (human/mouse/rat mature H-Ras4A, p21), 21.66 kDa (human/mouse/rat K-Ras4A pro-form), 21.17 kDa (human/mouse/rat mature K-Ras4A), 21.23/21.20/21.24 kDa (human/mouse/rat N-Ras pro-form), 20.90/20.90/20.91 kDa (human/mouse/rat mature N-Ras) calculated.
Physical form
Protein G purified.
Purified mouse IgG2a Alexa Fluor™ 555 conjugate in buffer containing PBS with 1.5% BSA and 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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