Skip to Content
Merck
  • Preparation, physical-chemical and biological characterization of chitosan nanoparticles loaded with lysozyme.

Preparation, physical-chemical and biological characterization of chitosan nanoparticles loaded with lysozyme.

International journal of biological macromolecules (2014-03-26)
Anna Maria Piras, Giuseppantonio Maisetta, Stefania Sandreschi, Semih Esin, Matteo Gazzarri, Giovanna Batoni, Federica Chiellini
ABSTRACT

A commercially available chitosan (CS) was employed in the formulation of nanoparticles loaded with lysozyme (LZ) as antimicrobial protein drug model. Due to the variability of commercially available batches of chitosans and to the strict dependence of their physical and biological properties to the molecular weight (Mw) and deacetylation degree (DD) of the material, the CS was fully characterized resulting in weight-average molecular weight of 108,120g/mol and DD of 92%. LZ-loaded nanoparticles (LZ-NPs) of 150nm diameter were prepared by inotropic gelation. The nanoparticles were effectively preserving the antibacterial activity of the loaded enzyme, which was slowly released over 3 weeks in vitro and remained active toward Staphylococcus epidermidis up to 5 days of incubation. Beyond the intrinsic antibacterial activity of CS and LZ, the LZ-NPs evidenced a sustained antibacterial activity that resulted in about 2 log reduction of the number of viable S. epidermidis compared to plain CS nanoparticles. Furthermore, the LZ-NPs showed a full in vitro cytocompatibility toward murine fibroblasts and, in addition to the potential antimicrobial applications of the developed system, the proposed study could serve as an optimal model for development of CS nanoparticles carrying antimicrobial peptides for biomedical applications.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Acetic acid-12C2, 99.9 atom % 12C
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
D-(+)-Glucosamine hydrochloride, ≥99%, BioReagent, suitable for cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Potassium bromide, BioXtra, ≥99.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
D-(+)-Glucosamine hydrochloride, ≥99% (HPLC), powder
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, natural, ≥99.5%, FG
SKU
Pack Size
Availability
Price
Quantity
Supelco
Acetic acid, analytical standard
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Potassium bromide, BioUltra, ≥99.5% (AT)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, for luminescence, BioUltra, ≥99.5% (GC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Potassium bromide, anhydrous, powder, 99.95% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, ≥99.5%, FCC, FG
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Potassium bromide, anhydrous, powder, 99.999% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Potassium bromide, anhydrous, free-flowing, Redi-Dri, ACS reagent, ≥99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid solution, suitable for HPLC
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium triphosphate pentabasic, purum p.a., ≥98.0% (T)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, glacial, ACS reagent, ≥99.7%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Potassium bromide, ReagentPlus®, ≥99.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, glacial, puriss., 99-100%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, glacial, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, 99.8-100.5%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Potassium bromide, anhydrous, free-flowing, Redi-Dri, ReagentPlus®, ≥99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, glacial, ReagentPlus®, ≥99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Potassium bromide, FT-IR grade, ≥99% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, glacial, ≥99.99% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Potassium bromide, ACS reagent, ≥99.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, glacial, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium tripolyphosphate, technical grade, 85%
SKU
Pack Size
Availability
Price
Quantity
Supelco
5α-Androstan-17β-ol-3-one, VETRANAL®, analytical standard
SKU
Pack Size
Availability
Price
Quantity
Millipore
Bifido Selective Supplement B, suitable for microbiology
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
5α-Androstan-17β-ol-3-one, purum, ≥99.0% (TLC)
SKU
Pack Size
Availability
Price
Quantity
Supelco
Glucosamine hydrochloride, Pharmaceutical Secondary Standard; Certified Reference Material
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
5α-Androstan-17β-ol-3-one, ≥97.5%
SKU
Pack Size
Availability
Price
Quantity