581343-U

Ascentis^® C18 (5 µm) HPLC Columns

L × I.D. 25 cm × 10 mm, HPLC Column

• Synonym(s): Ascentis RP18 HPLC Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: Ascentis^® C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm
• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L1 (Similar to Phenomenex Luna C18)
• product line: Ascentis^®
• feature: endcapped
• manufacturer/tradename: Ascentis^®
• packaging: 1 ea of
• extent of labeling: 25% Carbon loading
• parameter: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable
• L × I.D.: 25 cm × 10 mm
• surface area: 450 m²/g
• surface coverage: 3.7 μmol/m²
• impurities: <5 ppm metals
• matrix: fully porous particle; silica gel high purity, spherical
• matrix active group: C18 (octadecyl) phase
• particle size: 5 μm
• pore size: 100 Å
• operating pH range: 2-8
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.

Application

• A dilute-and-shoot liquid chromatography-tandem mass spectrometry method for urinary 18-hydroxycortisol quantification and its application in establishing reference intervals: This paper details the application of Ascentis^® C18 columns in a clinical setting to measure cortisol levels in urine. The method provides a fast, accurate, and reliable way to assess patient hormonal profiles, critical for diagnosing endocrine disorders (Zhao et al., 2022).


• A Comparative Study on Analysis of Ginsenosides in American Ginseng Root Residue by HPLC-DAD-ESI-MS and UPLC-HRMS-MS/MS: Utilizing Ascentis^® C18, this study compares different analytical methods for quantifying ginsenosides. It showcases the column′s adaptability and precision in natural product research, essential for ensuring the quality and efficacy of herbal medicines (Hsu et al., 2022).


• Validation of a Rapid and Easy-to-Apply Method to Simultaneously Quantify Co-Loaded Dexamethasone and Melatonin PLGA Microspheres by HPLC-UV: Highlighting the Ascentis^® C18′s effectiveness in pharmaceutical formulations, this research demonstrates its capacity to analyze complex drug delivery systems, contributing to the development of more effective therapeutic solutions (Brugnera et al., 2022).


• Simultaneous determination of ivermectin, clorsulon and their related substances in an injectable finished product by a stability-indicating RP-HPLC method: This publication illustrates the application of the Ascentis^® C18 in veterinary medicine, providing crucial insights into drug stability and composition which are vital for regulatory compliance and animal safety (Padivitage et al., 2022).

Features and Benefits

• Excellent retention
• Symmetric peak shape
• High reproducibility
• Complete LC-MS compatibility

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable